PMID- 22288597
OWN - NLM
STAT- MEDLINE
DCOM- 20120322
LR  - 20221207
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 167
IP  - 3
DP  - 2012 Mar
TI  - Induction of elastin expression in vascular endothelial cells relates to 
      hepatoportal sclerosis in idiopathic portal hypertension: possible link to serum 
      anti-endothelial cell antibodies.
PG  - 532-42
LID - 10.1111/j.1365-2249.2011.04530.x [doi]
AB  - Hepatoportal sclerosis accompanied by dense elastic fibre deposition is generally 
      regarded as the primary lesion in the development of idiopathic portal 
      hypertension (IPH). This study was performed to clarify the mechanism of elastic 
      fibre deposition in the peripheral portal tracts of IPH liver in relation to 
      serum anti-endothelial cell antibodies (AECA). In-vitro experiments were 
      performed using human dermal microvascular endothelial cells (HMVEC) and 
      patients' sera. The presence of serum AECA was assayed by a cell-based 
      enzyme-linked immunosorbent assay (ELISA) using HMVEC. Immunohistochemical 
      analysis of elastin was performed using liver tissue sections of IPH patients. 
      IPH sera contained one or more AECA that could bind to the vascular endothelial 
      cells of the peripheral portal tracts of the liver. When the value of AECA 
      greater than the mean +/- 2 standard deviations of healthy controls was regarded as 
      positive, the positive detection rate of either immunoglobulin (Ig)G, IgA or IgM 
      AECA in IPH sera was 30% (10 of 33 cases). IPH sera induced the expression of 
      elastin in HMVEC, which appeared to be associated with the presence of AECA. 
      Apoptosis was also induced in HMVEC by the stimulation with IPH sera. In vivo, 
      elastin expression was observed in the endothelial cells of the peripheral portal 
      tracts of IPH livers in a proportion of cases. The disease pathogenesis of IPH 
      seems to be heterogeneous, and this study elucidated a possible contribution of 
      the induction of elastin expression in the portal vessels to hepatoportal 
      sclerosis of IPH, which might be linked to serum AECA as a causative factor.
CI  - (c) 2011 The Authors. Clinical and Experimental Immunology (c) 2011 British Society 
      for Immunology.
FAU - Sato, Y
AU  - Sato Y
AD  - Department of Human Pathology, Kanazawa University Graduate School of Medicine, 
      Kanazawa, Japan.
FAU - Ren, X S
AU  - Ren XS
FAU - Harada, K
AU  - Harada K
FAU - Sasaki, M
AU  - Sasaki M
FAU - Morikawa, H
AU  - Morikawa H
FAU - Shiomi, S
AU  - Shiomi S
FAU - Honda, M
AU  - Honda M
FAU - Kaneko, S
AU  - Kaneko S
FAU - Nakanuma, Y
AU  - Nakanuma Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Autoantibodies)
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 9007-58-3 (Elastin)
SB  - IM
MH  - Apoptosis
MH  - Autoantibodies/*blood
MH  - Base Sequence
MH  - Case-Control Studies
MH  - Cells, Cultured
MH  - DNA Primers/genetics
MH  - Elastin/*biosynthesis/genetics
MH  - Endothelial Cells/*immunology/*metabolism
MH  - Humans
MH  - Hypertension, Portal/*etiology/immunology/metabolism/pathology
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Liver Cirrhosis/*etiology/immunology/metabolism/pathology
MH  - Pancytopenia/*etiology/immunology/metabolism/pathology
MH  - Portal Vein/*pathology
MH  - RNA, Messenger/genetics/metabolism
MH  - Sclerosis
MH  - Splenomegaly/*etiology/immunology/metabolism/pathology
MH  - Idiopathic Noncirrhotic Portal Hypertension
PMC - PMC3374286
EDAT- 2012/02/01 06:00
MHDA- 2012/03/23 06:00
PMCR- 2013/03/01
CRDT- 2012/02/01 06:00
PHST- 2012/02/01 06:00 [entrez]
PHST- 2012/02/01 06:00 [pubmed]
PHST- 2012/03/23 06:00 [medline]
PHST- 2013/03/01 00:00 [pmc-release]
AID - 10.1111/j.1365-2249.2011.04530.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2012 Mar;167(3):532-42. doi: 10.1111/j.1365-2249.2011.04530.x.