PMID- 22290765
OWN - NLM
STAT- MEDLINE
DCOM- 20120807
LR  - 20161125
IS  - 1098-2396 (Electronic)
IS  - 0887-4476 (Linking)
VI  - 66
IP  - 6
DP  - 2012 Jun
TI  - Test-retest stability of cerebral mGluR(5) quantification using [(1)(1)C]ABP688 and 
      positron emission tomography in rats.
PG  - 552-60
LID - 10.1002/syn.21542 [doi]
AB  - This study evaluates the reproducibility of the quantification of metabotropic 
      glutamate receptor type 5 (mGluR(5)) densities in rats using the PET radiotracer 
      [(1)(1)C]ABP688 and pharmacokinetic models that are based on an input function, which 
      is derived from a reference tissue. Seven rats underwent dynamic PET scans (60 
      min) after bolus injection of [(1)(1)C]ABP688. Kinetic analyses included: binding 
      potential (BP(ND) ) determined by calculating (a) the simplified reference tissue 
      model (SRTM) and (b) its two-steps simplified version (SRTM2); (c) multilinear 
      reference tissue model (MRTM) and (d) its 2-parameter version (MRTM2); (e) 
      noninvasive graphical analysis (NIGA). Parametric images were generated 
      representing BP(ND) by the MRTM2 model. BP(ND) determinations were reproducible 
      with low to acceptable variability ranging from 5 to 10% and reproducibility 
      scores (intraclass correlation coefficient) between 0.51 and 0.88. The 
      pharmacokinetic model that showed lowest overall variability was the SRTM. In 
      contrast, the use of the NIGA was associated with significantly lower 
      reproducibility scores. Comparison of parametric images revealed no significant 
      bias between test and retest measurements and is therefore suitable to compare 
      groups at voxel levels. In conclusion, our results suggest that noninvasive 
      quantification of [(1)(1)C]ABP688 imaging is reproducible and reliable for PET 
      studies of the cerebral mGluR(5) in rats.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Elmenhorst, David
AU  - Elmenhorst D
AD  - Translational Neuroimaging Laboratory, McGill Center for Studies in Aging, 
      Douglas Research Institute, Montreal, Quebec, Canada.
FAU - Aliaga, Antonio
AU  - Aliaga A
FAU - Bauer, Andreas
AU  - Bauer A
FAU - Rosa-Neto, Pedro
AU  - Rosa-Neto P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120316
PL  - United States
TA  - Synapse
JT  - Synapse (New York, N.Y.)
JID - 8806914
RN  - 0 (3-(6-methylpyridin-2-ylethynyl)cyclohex-2-enone-O-methyloxime)
RN  - 0 (Carbon Radioisotopes)
RN  - 0 (Oximes)
RN  - 0 (Pyridines)
RN  - 0 (Receptor, Metabotropic Glutamate 5)
RN  - 0 (Receptors, Metabotropic Glutamate)
SB  - IM
MH  - Algorithms
MH  - Animals
MH  - Brain/diagnostic imaging
MH  - *Brain Chemistry
MH  - Carbon Radioisotopes/chemistry
MH  - Image Interpretation, Computer-Assisted
MH  - Kinetics
MH  - Male
MH  - Oximes/*analysis/chemistry
MH  - Positron-Emission Tomography/*methods
MH  - Pyridines/*analysis/chemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, Metabotropic Glutamate 5
MH  - Receptors, Metabotropic Glutamate/*analysis
MH  - Reproducibility of Results
EDAT- 2012/02/01 06:00
MHDA- 2012/08/08 06:00
CRDT- 2012/02/01 06:00
PHST- 2011/12/06 00:00 [received]
PHST- 2012/01/24 00:00 [accepted]
PHST- 2012/02/01 06:00 [entrez]
PHST- 2012/02/01 06:00 [pubmed]
PHST- 2012/08/08 06:00 [medline]
AID - 10.1002/syn.21542 [doi]
PST - ppublish
SO  - Synapse. 2012 Jun;66(6):552-60. doi: 10.1002/syn.21542. Epub 2012 Mar 16.