PMID- 22291056
OWN - NLM
STAT- MEDLINE
DCOM- 20120403
LR  - 20211021
IS  - 1945-239X (Electronic)
IS  - 0021-9665 (Print)
IS  - 0021-9665 (Linking)
VI  - 50
IP  - 1
DP  - 2012 Jan
TI  - A high-performance liquid chromatographic-tandem mass spectrometric method for 
      the determination of ethyl glucuronide and ethyl sulfate in urine validated 
      according to forensic guidelines.
PG  - 51-6
LID - 10.1093/chromsci/bmr012 [doi]
AB  - Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are powerful markers for alcohol 
      intake and abuse. Several analytical procedures for the quantification of EtG and 
      EtG in serum and urine have been developed so far. Many of the published methods 
      show limits of detections (LODs) or limits of quantifications (LOQs) for EtG in 
      urine within the range of 0.1 mg/L or higher. Since this is the actual cutoff 
      value for proving abstinence in Germany, problems may occur if urine samples are 
      highly diluted. In this paper, the validation of a highly sensitive, fast and 
      simple LC-MS-MS for the determination of EtG and EtS in urine is described. The 
      calibration curves for EtG and EtS is linear over the whole range (0.025-2.0 
      mg/L). Very low detection limits can be achieved (LOD: EtG 0.005 mg/L, EtS 0.005 
      mg/L; and LOQ: EtG 0.019 mg/L, EtS 0.015 mg/L). All data for selectivity, 
      precision and accuracy, recovery, as well as for the processed sample and the 
      freeze/thaw stability, comply with the guidelines of the German Society of 
      Toxicological and Forensic Chemistry. Strong matrix-related effects can be 
      compensated for by using an internal standard. Finally, the applicability of the 
      procedure is proven by analysis of 87 human urine samples and by successful 
      participation in interlaboratory comparison tests.
CI  - (c) The Author [2011]. Published by Oxford University Press. All rights reserved.
FAU - Albermann, M E
AU  - Albermann ME
AD  - Institute of Forensic Medicine, University Hospital Bonn, Stiftsplatz 12, 53111 
      Bonn, Germany. elena.albermann@uni-bonn.de
FAU - Musshoff, F
AU  - Musshoff F
FAU - Madea, B
AU  - Madea B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Chromatogr Sci
JT  - Journal of chromatographic science
JID - 0173225
RN  - 0 (Glucuronates)
RN  - 0 (Sulfuric Acid Esters)
RN  - 17685-04-0 (ethyl glucuronide)
RN  - K0FO4VFA7I (diethyl sulfate)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drug Stability
MH  - Forensic Sciences/methods/*standards
MH  - Glucuronates/*urine
MH  - Humans
MH  - Limit of Detection
MH  - Reproducibility of Results
MH  - Sulfuric Acid Esters/*urine
MH  - Tandem Mass Spectrometry/*methods
PMC - PMC3252119
EDAT- 2012/02/01 06:00
MHDA- 2012/04/04 06:00
PMCR- 2013/01/01
CRDT- 2012/02/01 06:00
PHST- 2012/02/01 06:00 [entrez]
PHST- 2012/02/01 06:00 [pubmed]
PHST- 2012/04/04 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - bmr012 [pii]
AID - 10.1093/chromsci/bmr012 [doi]
PST - ppublish
SO  - J Chromatogr Sci. 2012 Jan;50(1):51-6. doi: 10.1093/chromsci/bmr012.