PMID- 22291966
OWN - NLM
STAT- MEDLINE
DCOM- 20120604
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 1
DP  - 2012
TI  - Chemically-induced cancers do not originate from bone marrow-derived cells.
PG  - e30493
LID - 10.1371/journal.pone.0030493 [doi]
LID - e30493
AB  - BACKGROUND: The identification and characterization of cancer stem cells (CSCs) 
      is imperative to understanding the mechanism of cancer pathogenesis. Growing 
      evidence suggests that CSCs play critical roles in the development and 
      progression of cancer. However, controversy exists as to whether CSCs arise from 
      bone marrow-derived cells (BMDCs). METHODOLOGY AND PRINCIPAL FINDINGS: In the 
      present study, n-nitrosodiethylamine (DEN) was used to induce tumor formation in 
      female mice that received bone marrow from male mice. Tumor formation was induced 
      in 20/26 mice, including 12 liver tumors, 6 lung tumors, 1 bladder tumor and 1 
      nasopharyngeal tumor. Through comparison of fluorescence in situ hybridization 
      (FISH) results in corresponding areas from serial tumor sections stained with 
      H&E, we determined that BMDCs were recruited to both tumor tissue and normal 
      surrounding tissue at a very low frequency (0.2-1% in tumors and 0-0.3% in normal 
      tissues). However, approximately 3-70% of cells in the tissues surrounding the 
      tumor were BMDCs, and the percentage of BMDCs was highly associated with the 
      inflammatory status of the tissue. In the present study, no evidence was found to 
      support the existence of fusion cells formed form BMDCs and tissue-specific stem 
      cells. CONCLUSIONS: In summary, our data suggest that although BMDCs may 
      contribute to tumor progression, they are unlike to contribute to tumor 
      initiation.
FAU - Lin, Hui
AU  - Lin H
AD  - Department of General Surgery, Sir RunRun Shaw Hospital, School of Medicine, 
      Zhejiang University, Hangzhou, China.
FAU - Hu, Liang
AU  - Hu L
FAU - Chen, Leilei
AU  - Chen L
FAU - Yu, Hong
AU  - Yu H
FAU - Wang, Qi
AU  - Wang Q
FAU - Chen, Ping
AU  - Chen P
FAU - Hu, Xiao-Tong
AU  - Hu XT
FAU - Cai, Xiu-Jun
AU  - Cai XJ
FAU - Guan, Xin-Yuan
AU  - Guan XY
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120124
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Carcinogens)
RN  - 3IQ78TTX1A (Diethylnitrosamine)
SB  - IM
MH  - Abnormal Karyotype/chemically induced
MH  - Animals
MH  - Bone Marrow Cells/*pathology
MH  - Bone Marrow Transplantation/adverse effects/physiology
MH  - *Carcinogens
MH  - Cell Transformation, Neoplastic/*pathology
MH  - Diethylnitrosamine
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neoplasms/*chemically induced/epidemiology/pathology
MH  - Neoplastic Stem Cells/*drug effects/*pathology
MH  - Sex Chromosome Aberrations/chemically induced
MH  - Sex Chromosomes/metabolism
PMC - PMC3265477
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/02/01 06:00
MHDA- 2012/06/05 06:00
PMCR- 2012/01/24
CRDT- 2012/02/01 06:00
PHST- 2011/11/16 00:00 [received]
PHST- 2011/12/16 00:00 [accepted]
PHST- 2012/02/01 06:00 [entrez]
PHST- 2012/02/01 06:00 [pubmed]
PHST- 2012/06/05 06:00 [medline]
PHST- 2012/01/24 00:00 [pmc-release]
AID - PONE-D-11-22911 [pii]
AID - 10.1371/journal.pone.0030493 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(1):e30493. doi: 10.1371/journal.pone.0030493. Epub 2012 Jan 24.