PMID- 22291969
OWN - NLM
STAT- MEDLINE
DCOM- 20120604
LR  - 20220331
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 1
DP  - 2012
TI  - Inhibition of histone deacetylase activity in human endometrial stromal cells 
      promotes extracellular matrix remodelling and limits embryo invasion.
PG  - e30508
LID - 10.1371/journal.pone.0030508 [doi]
LID - e30508
AB  - Invasion of the trophoblast into the maternal decidua is regulated by both the 
      trophoectoderm and the endometrial stroma, and entails the action of tissue 
      remodeling enzymes. Trophoblast invasion requires the action of 
      metalloproteinases (MMPs) to degrade extracellular matrix (ECM) proteins and in 
      turn, decidual cells express tissue inhibitors of MMPs (TIMPs). The balance 
      between these promoting and restraining factors is a key event for the successful 
      outcome of pregnancy. Gene expression is post-transcriptionally regulated by 
      histone deacetylases (HDACs) that unpacks condensed chromatin activating gene 
      expression. In this study we analyze the effect of histone acetylation on the 
      expression of tissue remodeling enzymes and activity of human endometrial stromal 
      cells (hESCs) related to trophoblast invasion control. Treatment of hESCs with 
      the HDAC inhibitor trichostatin A (TSA) increased the expression of TIMP-1 and 
      TIMP-3 while decreased MMP-2, MMP-9 and uPA and have an inhibitory effect on 
      trophoblast invasion. Moreover, histone acetylation is detected at the promoters 
      of TIMP-1 and TIMP-3 genes in TSA-treated. In addition, in an in vitro 
      decidualized hESCs model, the increase of TIMP-1 and TIMP-3 expression is 
      associated with histone acetylation at the promoters of these genes. Our results 
      demonstrate that histone acetylation disrupt the balance of ECM modulators 
      provoking a restrain of trophoblast invasion. These findings are important as an 
      epigenetic mechanism that can be used to control trophoblast invasion.
FAU - Estella, Carlos
AU  - Estella C
AD  - Fundacion Instituto Valenciano de Infertilidad, Valencia University, and 
      Instituto Universitario IVI/INCLIVA, Valencia, Spain.
FAU - Herrer, Isabel
AU  - Herrer I
FAU - Atkinson, Stuart P
AU  - Atkinson SP
FAU - Quinonero, Alicia
AU  - Quinonero A
FAU - Martinez, Sebastian
AU  - Martinez S
FAU - Pellicer, Antonio
AU  - Pellicer A
FAU - Simon, Carlos
AU  - Simon C
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20120126
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Histone Deacetylase Inhibitors)
RN  - 0 (Hydroxamic Acids)
RN  - 0 (TIMP1 protein, human)
RN  - 0 (TIMP3 protein, human)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-3)
RN  - 3X2S926L3Z (trichostatin A)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - EC 3.4.24.24 (MMP2 protein, human)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Adult
MH  - Cells, Cultured
MH  - Down-Regulation/drug effects/genetics
MH  - Embryo Implantation/*drug effects/genetics/physiology
MH  - Endometrium/*drug effects/enzymology/metabolism
MH  - Extracellular Matrix/*drug effects/metabolism
MH  - Female
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Histone Deacetylase Inhibitors/*pharmacology
MH  - Humans
MH  - Hydroxamic Acids/pharmacology
MH  - Matrix Metalloproteinase 2/genetics/metabolism
MH  - Matrix Metalloproteinase 9/genetics/metabolism
MH  - Pregnancy
MH  - Stromal Cells/*drug effects/enzymology/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-1/genetics/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-3/genetics/metabolism
MH  - Urokinase-Type Plasminogen Activator/genetics/metabolism
MH  - Young Adult
PMC - PMC3266920
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/02/01 06:00
MHDA- 2012/06/05 06:00
PMCR- 2012/01/26
CRDT- 2012/02/01 06:00
PHST- 2011/11/03 00:00 [received]
PHST- 2011/12/22 00:00 [accepted]
PHST- 2012/02/01 06:00 [entrez]
PHST- 2012/02/01 06:00 [pubmed]
PHST- 2012/06/05 06:00 [medline]
PHST- 2012/01/26 00:00 [pmc-release]
AID - PONE-D-11-22064 [pii]
AID - 10.1371/journal.pone.0030508 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(1):e30508. doi: 10.1371/journal.pone.0030508. Epub 2012 Jan 26.