PMID- 22293971
OWN - NLM
STAT- MEDLINE
DCOM- 20120611
LR  - 20220316
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 402
IP  - 9
DP  - 2012 Mar
TI  - Stable isotope dilution assay for the accurate determination of mycotoxins in 
      maize by UHPLC-MS/MS.
PG  - 2675-86
LID - 10.1007/s00216-012-5757-5 [doi]
AB  - A fast, easy-to-handle and cost-effective analytical method for 11 mycotoxins 
      currently regulated in maize and other cereal-based food products in Europe was 
      developed and validated for maize. The method is based on two extraction steps 
      using different acidified acetonitrile-water mixtures. Separation is achieved 
      using ultrahigh-performance liquid chromatography (UHPLC) by a linear 
      water-methanol gradient. After electrospray ionisation, tandem mass spectrometric 
      detection is performed in dynamic multiple reaction monitoring mode. Since 
      accurate mass spectrometric quantification is hampered by matrix effects, 
      uniformly [(13)C]-labelled mycotoxins for each of the 11 compounds were added to 
      the sample extracts prior to UHPLC-MS/MS analysis. Method performance parameters 
      were obtained by spiking blank maize samples with mycotoxins before as well as 
      after extraction on six levels in triplicates. The twofold extraction led to 
      total recoveries of the extraction steps between 97% and 111% for all target 
      analytes, including fumonisins. The [(13)C]-labelled internal standards 
      efficiently compensated all matrix effects in electrospray ionisation, leading to 
      apparent recoveries between 88% and 105% with reasonable additional costs. The 
      relative standard deviations of the whole method were between 4% and 11% for all 
      analytes. The trueness of the method was verified by the measurement of several 
      maize test materials with well-characterized concentrations. In conclusion, the 
      developed method is capable of determining all regulated mycotoxins in maize and 
      presuming similar matrix effects and extraction recovery also in other 
      cereal-based foods.
FAU - Varga, Elisabeth
AU  - Varga E
AD  - Christian Doppler Laboratory for Mycotoxin Metabolism, Department for 
      Agrobiotechnology, University of Natural Resources and Life Sciences Vienna, 
      Tulln, Austria.
FAU - Glauner, Thomas
AU  - Glauner T
FAU - Koppen, Robert
AU  - Koppen R
FAU - Mayer, Katharina
AU  - Mayer K
FAU - Sulyok, Michael
AU  - Sulyok M
FAU - Schuhmacher, Rainer
AU  - Schuhmacher R
FAU - Krska, Rudolf
AU  - Krska R
FAU - Berthiller, Franz
AU  - Berthiller F
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Carbon Isotopes)
RN  - 0 (Mycotoxins)
SB  - IM
MH  - Carbon Isotopes/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Food Contamination/*analysis
MH  - *Indicator Dilution Techniques
MH  - Mycotoxins/*analysis
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Tandem Mass Spectrometry/methods
MH  - Zea mays/*chemistry
PMC - PMC3292730
EDAT- 2012/02/02 06:00
MHDA- 2012/06/12 06:00
PMCR- 2012/02/02
CRDT- 2012/02/02 06:00
PHST- 2011/12/09 00:00 [received]
PHST- 2012/01/16 00:00 [accepted]
PHST- 2012/01/13 00:00 [revised]
PHST- 2012/02/02 06:00 [entrez]
PHST- 2012/02/02 06:00 [pubmed]
PHST- 2012/06/12 06:00 [medline]
PHST- 2012/02/02 00:00 [pmc-release]
AID - 5757 [pii]
AID - 10.1007/s00216-012-5757-5 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2012 Mar;402(9):2675-86. doi: 10.1007/s00216-012-5757-5.