PMID- 22294105
OWN - NLM
STAT- MEDLINE
DCOM- 20120813
LR  - 20211021
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 39
IP  - 6
DP  - 2012 Jun
TI  - Functional study of immature dendritic cells co-transfected with IL-10 and 
      TGF-beta 1 genes in vitro.
PG  - 6633-9
LID - 10.1007/s11033-012-1468-4 [doi]
AB  - Dendritic cells (DC) have important functions in T cell immunity and T cell 
      tolerance. Previous studies suggest that immature dendritic cells (imDCs) might 
      be involved in the induction of peripheral T cell tolerance. While interleukin-10 
      (IL-10) functions at different levels of the immune response, transforming growth 
      factor-beta 1 (TGF-beta 1) is considered to be a key factor in immune tolerance. 
      In this study, we investigated the effects of immature DC (imDC) co-transfected 
      with IL-10 and TGF-beta 1 genes (IL-10-TGF-beta 1-imDC) on inducing immune 
      tolerance. Moreover, we compared the effects of IL-10-TGF-beta 1-imDC with IL-10 
      transfected imDC (IL-10-imDC) and TGF-beta 1-transfected imDC (TGF-beta 1-imDC), 
      respectively. IL-10-TGF-beta 1-imDC resulted in the down-regulation of MHC class 
      II, CD80 and CD86. IL-10-TGF-beta 1-imDC could induce T cell hyporesponsiveness, 
      and was reluctant to proliferate. IL-10-TGF-beta 1-imDC was more effective than 
      IL-10-imDC and TGF-beta 1-imDC, respectively. In summary, co-expression of IL-10 
      and TGF-beta 1 affected the immunity of imDCs and enhanced their tolerogenicity. 
      It might be a promising therapy for donor-specific tolerance after organ 
      transplantation.
FAU - Chen, Lihong
AU  - Chen L
AD  - Department of Pathology, Fujian Medical University, Fuzhou, Fujian, China.
FAU - Qiu, Minglian
AU  - Qiu M
FAU - He, Wubing
AU  - He W
FAU - Huang, Aimin
AU  - Huang A
FAU - Liu, Jingfeng
AU  - Liu J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Cd86 protein, rat)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - B7-1 Antigen/genetics/metabolism
MH  - B7-2 Antigen/genetics/metabolism
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Dendritic Cells/metabolism/*physiology
MH  - Gene Expression
MH  - Histocompatibility Antigens Class II/genetics/metabolism
MH  - Immunomodulation/genetics
MH  - Interleukin-10/*biosynthesis/genetics
MH  - Male
MH  - Rats, Inbred Lew
MH  - Recombinant Proteins/biosynthesis/genetics
MH  - T-Lymphocytes/physiology
MH  - Transfection
MH  - Transforming Growth Factor beta1/*biosynthesis/genetics
EDAT- 2012/02/02 06:00
MHDA- 2012/08/14 06:00
CRDT- 2012/02/02 06:00
PHST- 2011/12/15 00:00 [received]
PHST- 2012/01/23 00:00 [accepted]
PHST- 2012/02/02 06:00 [entrez]
PHST- 2012/02/02 06:00 [pubmed]
PHST- 2012/08/14 06:00 [medline]
AID - 10.1007/s11033-012-1468-4 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2012 Jun;39(6):6633-9. doi: 10.1007/s11033-012-1468-4.