PMID- 22294982
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20220129
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 2
DP  - 2011
TI  - Similar diversity of alphaproteobacteria and nitrogenase gene amplicons on two 
      related sphagnum mosses.
PG  - 275
LID - 10.3389/fmicb.2011.00275 [doi]
LID - 275
AB  - Sphagnum mosses represent a main vegetation component in ombrotrophic wetlands. 
      They harbor a specific and diverse microbial community with essential functions 
      for the host. To understand the extend of host specificity and impact of 
      environment, Sphagnum fallax and Sphagnum angustifolium, two phylogenetically 
      closely related species, which show distinct habitat preference with respect to 
      the nutrient level, were analyzed by a multifaceted approach. Microbial 
      fingerprints obtained by PCR-single-strand conformation polymorphism of 16S rRNA 
      and nitrogenase-encoding (nifH) genes were highly similar for both Sphagnum 
      species. Similarity was confirmed for colonization patterns obtained by 
      fluorescence in situ hybridization (FISH) coupled with confocal laser scanning 
      microscopy (CLSM): Alphaproteobacteria were the main colonizers inside the 
      hyaline cells of Sphagnum leaves. A deeper survey of Alphaproteobacteria by 16S 
      rRNA gene amplicon sequencing reveals a high diversity with Acidocella, 
      Acidisphaera, Rhodopila, and Phenylobacterium as major genera for both mosses. 
      Nitrogen fixation is an important function of Sphagnum-associated bacteria, which 
      is fulfilled by microbial communities of Sphagna in a similar way. NifH libraries 
      of Sphagnum-associated microbial communities were characterized by high diversity 
      and abundance of Alphaproteobacteria but contained also diverse amplicons of 
      other taxa, e.g., Cyanobacteria and Deltaproteobacteria. Statistically 
      significant differences between the microbial communities of both Sphagnum 
      species could not be discovered in any of the experimental approach. Our results 
      show that the same close relationship, which exists between the physical, 
      morphological, and chemical characteristics of Sphagnum mosses and the ecology 
      and function of bog ecosystems, also connects moss plantlets with their 
      associated bacterial communities.
FAU - Bragina, Anastasia
AU  - Bragina A
AD  - Institute of Environmental Biotechnology, Graz University of Technology Graz, 
      Austria.
FAU - Maier, Stefanie
AU  - Maier S
FAU - Berg, Christian
AU  - Berg C
FAU - Muller, Henry
AU  - Muller H
FAU - Chobot, Vladimir
AU  - Chobot V
FAU - Hadacek, Franz
AU  - Hadacek F
FAU - Berg, Gabriele
AU  - Berg G
LA  - eng
GR  - I 183/FWF_/Austrian Science Fund FWF/Austria
PT  - Journal Article
DEP - 20120110
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC3261640
OTO - NOTNLM
OT  - Alphaproteobacteria
OT  - FISH-CLSM
OT  - SSCP fingerprints
OT  - Sphagnum angustifolium
OT  - Sphagnum fallax
OT  - amplicon library
OT  - nitrogenase
EDAT- 2012/02/02 06:00
MHDA- 2012/02/02 06:01
PMCR- 2012/01/10
CRDT- 2012/02/02 06:00
PHST- 2011/11/11 00:00 [received]
PHST- 2011/12/22 00:00 [accepted]
PHST- 2012/02/02 06:00 [entrez]
PHST- 2012/02/02 06:00 [pubmed]
PHST- 2012/02/02 06:01 [medline]
PHST- 2012/01/10 00:00 [pmc-release]
AID - 10.3389/fmicb.2011.00275 [doi]
PST - epublish
SO  - Front Microbiol. 2012 Jan 10;2:275. doi: 10.3389/fmicb.2011.00275. eCollection 
      2011.