PMID- 22297041
OWN - NLM
STAT- MEDLINE
DCOM- 20120718
LR  - 20211021
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 206
DP  - 2012 Mar 29
TI  - Acute intermittent hypoxia-induced expression of brain-derived neurotrophic 
      factor is disrupted in the brainstem of methyl-CpG-binding protein 2 null mice.
PG  - 1-6
LID - 10.1016/j.neuroscience.2012.01.017 [doi]
AB  - Rett syndrome is a neurodevelopmental disorder caused by loss-of-function 
      mutations in the gene encoding the transcription factor methyl-CpG-binding 
      protein 2 (MeCP2). One of its targets is the gene encoding brain-derived 
      neurotrophic factor (bdnf). In vitro studies using cultured neurons have produced 
      conflicting results with respect to the role of MeCP2 in BDNF expression. Acute 
      intermittent hypoxia (AIH) induces plasticity in the respiratory system 
      characterized by long-term facilitation of phrenic nerve amplitude. This paradigm 
      induces an increase in BDNF protein. We hypothesized that AIH leads to 
      augmentation of BDNF transcription in respiratory-related areas of the brainstem 
      and that MeCP2 is necessary for this process. Wild-type and mecp2 null 
      (mecp2(-/y)) mice were subjected to three 5-min episodes of exposure to 8% 
      O(2)/4% CO(2)/88% N(2), delivered at 5-min intervals. Normoxia control wild-type 
      and mecp2 null mice were exposed to room air for the total length of time, that 
      is, 30 min. Following a recovery in room air, the pons and medulla were rapidly 
      removed. Expression of BDNF protein and transcripts were determined by ELISA and 
      quantitative PCR, respectively. AIH induced a significant increase in BDNF 
      protein in the pons and medulla, and in mRNA transcript levels in the pons of 
      wild-type animals. In contrast, there were no significant changes in either BDNF 
      protein or transcripts in the pons or medulla of mice lacking MeCP2. The results 
      indicate that MeCP2 is required for regulation of BDNF expression by acute 
      intermittent hypoxia in vivo.
CI  - Copyright (c) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Vermehren-Schmaedick, A
AU  - Vermehren-Schmaedick A
AD  - Department of Integrative Biosciences, Oregon Health and Science University, 
      Portland, OR 97239, USA.
FAU - Jenkins, V K
AU  - Jenkins VK
FAU - Knopp, S J
AU  - Knopp SJ
FAU - Balkowiec, A
AU  - Balkowiec A
FAU - Bissonnette, J M
AU  - Bissonnette JM
LA  - eng
GR  - HL076113/HL/NHLBI NIH HHS/United States
GR  - R01 HL076113-05/HL/NHLBI NIH HHS/United States
GR  - R01 HL076113-05S1/HL/NHLBI NIH HHS/United States
GR  - R01 HL076113/HL/NHLBI NIH HHS/United States
GR  - R01 HL076113-03/HL/NHLBI NIH HHS/United States
GR  - R01 HL076113-04/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120118
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Methyl-CpG-Binding Protein 2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Brain Stem/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Hypoxia/*metabolism
MH  - Methyl-CpG-Binding Protein 2/deficiency/*metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - RNA, Messenger/analysis
MH  - Rett Syndrome/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC3293990
MID - NIHMS349463
EDAT- 2012/02/03 06:00
MHDA- 2012/07/19 06:00
PMCR- 2012/03/29
CRDT- 2012/02/03 06:00
PHST- 2011/09/30 00:00 [received]
PHST- 2011/12/20 00:00 [revised]
PHST- 2012/01/08 00:00 [accepted]
PHST- 2012/02/03 06:00 [entrez]
PHST- 2012/02/03 06:00 [pubmed]
PHST- 2012/07/19 06:00 [medline]
PHST- 2012/03/29 00:00 [pmc-release]
AID - S0306-4522(12)00039-5 [pii]
AID - 10.1016/j.neuroscience.2012.01.017 [doi]
PST - ppublish
SO  - Neuroscience. 2012 Mar 29;206:1-6. doi: 10.1016/j.neuroscience.2012.01.017. Epub 
      2012 Jan 18.