PMID- 22297554
OWN - NLM
STAT- MEDLINE
DCOM- 20121001
LR  - 20211021
IS  - 1573-7446 (Electronic)
IS  - 0165-7380 (Linking)
VI  - 36
IP  - 2
DP  - 2012 Jun
TI  - A transgenic Marc-145 cell line of piggyBac transposon-derived targeting shRNA 
      interference against porcine reproductive and respiratory syndrome virus.
PG  - 99-105
LID - 10.1007/s11259-012-9519-9 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) is now considered to be one 
      of the most important diseases in countries with intensive swine industries. The 
      two major membrane-associated proteins of porcine reproductive and respiratory 
      syndrome virus (PRRSV), GP5 and M (encoded by ORF5 and ORF6 genes, respectively), 
      are associated as disulfide-linked heterodimers (GP5/M) in the virus particle. In 
      this study, we designed 5 of the small hairpin RNAs (shRNAs) targeting the GP5 
      and M gene of PRRSV respectively, and investigated their inhibition to the 
      production of PRRSV. The highest activity displayed in shRNAs of the ORF6e 
      sequence (nts 261-279), which the inhibition rate reached was 99.09%. The result 
      suggests that RNAi technology might serve as a potential molecular strategy for 
      PRRSV therapy. Furthermore, the transgenic Marc-145 cell line of piggyBac 
      transposon-derived targeting shRNA interference against PRRS virus was 
      established. It presented stable inhibition to the replication and amplification 
      of PRRS. The work implied that shRNAs targeting the GP5 and M gene of PRRSV may 
      be used as potential RNA vaccines in vivo, and supplied the screening methods of 
      transformed pig embryonic fibroblast which are prerequisite for the 
      disease-resistant transgenic pigs to PRRS.
FAU - Zhou, Fang
AU  - Zhou F
AD  - Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal 
      Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
FAU - Liang, Shuang
AU  - Liang S
FAU - Chen, An-hui
AU  - Chen AH
FAU - Singh, Chabungbam Orville
AU  - Singh CO
FAU - Bhaskar, Roy
AU  - Bhaskar R
FAU - Niu, Yan-shan
AU  - Niu YS
FAU - Miao, Yun-gen
AU  - Miao YG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Vet Res Commun
JT  - Veterinary research communications
JID - 8100520
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Gene Expression Regulation, Viral/physiology
MH  - Plasmids
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - RNA Interference/*physiology
MH  - RNA, Small Interfering/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Swine
MH  - Transfection
MH  - Viral Envelope Proteins/genetics/*metabolism
EDAT- 2012/02/03 06:00
MHDA- 2012/10/02 06:00
CRDT- 2012/02/03 06:00
PHST- 2012/01/13 00:00 [accepted]
PHST- 2012/02/03 06:00 [entrez]
PHST- 2012/02/03 06:00 [pubmed]
PHST- 2012/10/02 06:00 [medline]
AID - 10.1007/s11259-012-9519-9 [doi]
PST - ppublish
SO  - Vet Res Commun. 2012 Jun;36(2):99-105. doi: 10.1007/s11259-012-9519-9.