PMID- 22298307
OWN - NLM
STAT- MEDLINE
DCOM- 20121011
LR  - 20211021
IS  - 1535-9484 (Electronic)
IS  - 1535-9476 (Print)
IS  - 1535-9476 (Linking)
VI  - 11
IP  - 6
DP  - 2012 Jun
TI  - Identification of candidate biomarkers for early detection of human lung squamous 
      cell cancer by quantitative proteomics.
PG  - M111.013946
LID - 10.1074/mcp.M111.013946 [doi]
LID - M111.013946
AB  - To discover novel biomarkers for early detection of human lung squamous cell 
      cancer (LSCC) and explore possible mechanisms of LSCC carcinogenesis, 
      iTRAQ-tagging combined with two dimensional liquid chromatography tandem MS 
      analysis was used to identify differentially expressed proteins in human 
      bronchial epithelial carcinogenic process using laser capture 
      microdissection-purified normal bronchial epithelium (NBE), squamous metaplasia 
      (SM), atypical hyperplasia (AH), carcinoma in situ (CIS) and invasive LSCC. As a 
      result, 102 differentially expressed proteins were identified, and three 
      differential proteins (GSTP1, HSPB1 and CKB) showing progressively expressional 
      changes in the carcinogenic process were selectively validated by Western 
      blotting. Immunohistochemistry was performed to detect the expression of the 
      three proteins in an independent set of paraffin-embedded archival specimens 
      including various stage tissues of bronchial epithelial carcinogenesis, and their 
      ability for early detection of LSCC was evaluated by receiver operating 
      characteristic analysis. The results showed that the combination of the three 
      proteins could perfectly discriminate NBE from preneoplastic lesions (SM, AH and 
      CIS) from invasive LSCC, achieving a sensitivity of 96% and a specificity of 92% 
      in discriminating NBE from preneoplatic lesions, a sensitivity of 100% and a 
      specificity of 98% in discriminating NBE from invasive LSCC, and a sensitivity of 
      92% and a specificity of 91% in discriminating preneoplastic lesions from 
      invasive LSCC, respectively. Furthermore, we knocked down GSTP1 in immortalized 
      human bronchial epithelial cell line 16HBE cells, and then measured their 
      susceptibility to carcinogen benzo(a)pyrene-induced cell transformation. The 
      results showed that GSTP1 knockdown significantly increased the efficiency of 
      benzo(a)pyrene-induced 16HBE cell transformation. The present data first time 
      show that GSTP1, HSPB1 and CKB are novel potential biomarkers for early detection 
      of LSCC, and GSTP1 down-regulation is involved in human bronchial epithelial 
      carcinogenesis.
FAU - Zeng, Gu-Qing
AU  - Zeng GQ
AD  - Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya 
      Hospital, Central South University, Changsha 410008, China.
FAU - Zhang, Pang-Fei
AU  - Zhang PF
FAU - Deng, Xingming
AU  - Deng X
FAU - Yu, Feng-Lei
AU  - Yu FL
FAU - Li, Cui
AU  - Li C
FAU - Xu, Yan
AU  - Xu Y
FAU - Yi, Hong
AU  - Yi H
FAU - Li, Mao-Yu
AU  - Li MY
FAU - Hu, Rong
AU  - Hu R
FAU - Zuo, Jian-Hong
AU  - Zuo JH
FAU - Li, Xin-Hui
AU  - Li XH
FAU - Wan, Xun-Xun
AU  - Wan XX
FAU - Qu, Jia-Quan
AU  - Qu JQ
FAU - He, Qiu-Yan
AU  - He QY
FAU - Li, Jian-Huang
AU  - Li JH
FAU - Ye, Xu
AU  - Ye X
FAU - Chen, Yu
AU  - Chen Y
FAU - Li, Jiao-Yang
AU  - Li JY
FAU - Xiao, Zhi-Qiang
AU  - Xiao ZQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120131
PL  - United States
TA  - Mol Cell Proteomics
JT  - Molecular & cellular proteomics : MCP
JID - 101125647
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (HSP27 Heat-Shock Proteins)
RN  - 0 (HSPB1 protein, human)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Molecular Chaperones)
RN  - EC 2.5.1.18 (GSTP1 protein, human)
RN  - EC 2.5.1.18 (Glutathione S-Transferase pi)
RN  - EC 2.7.3.2 (Creatine Kinase, BB Form)
SB  - IM
MH  - Amino Acid Sequence
MH  - Biomarkers, Tumor/chemistry/genetics/*metabolism
MH  - Bronchi/pathology
MH  - Cell Line
MH  - Cell Transformation, Neoplastic/chemically induced/metabolism
MH  - Cluster Analysis
MH  - Creatine Kinase, BB Form/chemistry/genetics/metabolism
MH  - *Early Detection of Cancer
MH  - Epithelial Cells/metabolism/pathology
MH  - Gene Expression
MH  - Glutathione S-Transferase pi/chemistry/genetics/metabolism
MH  - HSP27 Heat-Shock Proteins/chemistry/genetics/metabolism
MH  - Heat-Shock Proteins
MH  - Humans
MH  - Laser Capture Microdissection
MH  - Lung Neoplasms/diagnosis/*metabolism
MH  - Molecular Chaperones
MH  - Molecular Sequence Data
MH  - Neoplasms, Squamous Cell/diagnosis/*metabolism
MH  - Proteomics
MH  - ROC Curve
MH  - Statistics, Nonparametric
MH  - Tandem Mass Spectrometry
PMC - PMC3433890
EDAT- 2012/02/03 06:00
MHDA- 2012/10/12 06:00
PMCR- 2013/06/01
CRDT- 2012/02/03 06:00
PHST- 2012/02/03 06:00 [entrez]
PHST- 2012/02/03 06:00 [pubmed]
PHST- 2012/10/12 06:00 [medline]
PHST- 2013/06/01 00:00 [pmc-release]
AID - S1535-9476(20)30417-5 [pii]
AID - M111.013946 [pii]
AID - 10.1074/mcp.M111.013946 [doi]
PST - ppublish
SO  - Mol Cell Proteomics. 2012 Jun;11(6):M111.013946. doi: 10.1074/mcp.M111.013946. 
      Epub 2012 Jan 31.