PMID- 22298786
OWN - NLM
STAT- MEDLINE
DCOM- 20120516
LR  - 20240406
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 287
IP  - 13
DP  - 2012 Mar 23
TI  - Role of metalloproteases in vaccinia virus epitope processing for transporter 
      associated with antigen processing (TAP)-independent human leukocyte antigen 
      (HLA)-B7 class I antigen presentation.
PG  - 9990-10000
LID - S0021-9258(20)65412-6 [pii]
LID - 10.1074/jbc.M111.314856 [doi]
AB  - The transporter associated with antigen processing (TAP) translocates the viral 
      proteolytic peptides generated by the proteasome and other proteases in the 
      cytosol to the endoplasmic reticulum lumen. There, they complex with nascent 
      human leukocyte antigen (HLA) class I molecules, which are subsequently 
      recognized by the CD8(+) lymphocyte cellular response. However, individuals with 
      nonfunctional TAP complexes or tumor or infected cells with blocked TAP molecules 
      are able to present HLA class I ligands generated by TAP-independent processing 
      pathways. Herein, using a TAP-independent polyclonal vaccinia virus-polyspecific 
      CD8(+) T cell line, two conserved vaccinia-derived TAP-independent HLA-B*0702 
      epitopes were identified. The presentation of these epitopes in normal cells 
      occurs via complex antigen-processing pathways involving the proteasome and/or 
      different subsets of metalloproteinases (amino-, carboxy-, and endoproteases), 
      which were blocked in infected cells with specific chemical inhibitors. These 
      data support the hypothesis that the abundant cellular proteolytic systems 
      contribute to the supply of peptides recognized by the antiviral cellular immune 
      response, thereby facilitating immunosurveillance. These data may explain why 
      TAP-deficient individuals live normal life spans without any increased 
      susceptibility to viral infections.
FAU - Lorente, Elena
AU  - Lorente E
AD  - Instituto de Salud Carlos III, Centro Nacional de Microbiologia, 28220 
      Majadahonda (Madrid), Spain and.
FAU - Garcia, Ruth
AU  - Garcia R
AD  - Instituto de Salud Carlos III, Centro Nacional de Microbiologia, 28220 
      Majadahonda (Madrid), Spain and.
FAU - Mir, Carmen
AU  - Mir C
AD  - Instituto de Salud Carlos III, Centro Nacional de Microbiologia, 28220 
      Majadahonda (Madrid), Spain and.
FAU - Barriga, Alejandro
AU  - Barriga A
AD  - Instituto de Salud Carlos III, Centro Nacional de Microbiologia, 28220 
      Majadahonda (Madrid), Spain and.
FAU - Lemonnier, Francois A
AU  - Lemonnier FA
AD  - Unite d'Immunite Cellulaire Antivirale, Departement d'Immunologie, Institut 
      Pasteur, Paris Cedex 15, France.
FAU - Ramos, Manuel
AU  - Ramos M
AD  - Instituto de Salud Carlos III, Centro Nacional de Microbiologia, 28220 
      Majadahonda (Madrid), Spain and.
FAU - Lopez, Daniel
AU  - Lopez D
AD  - Instituto de Salud Carlos III, Centro Nacional de Microbiologia, 28220 
      Majadahonda (Madrid), Spain and. Electronic address: dlopez@isciii.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120201
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antigens, Ly)
RN  - 0 (Epitopes)
RN  - 0 (HLA-B7 Antigen)
RN  - 0 (Ly6a protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - EC 3.4.- (Metalloproteases)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
CIN - J Biol Chem. 2012 Aug 3;287(32):27047; author reply 27048. PMID: 22865894
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigens, Ly/genetics/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology/metabolism
MH  - Epitopes/genetics/*immunology
MH  - HLA-B7 Antigen/genetics/*immunology
MH  - Humans
MH  - Membrane Proteins/genetics/*immunology
MH  - Metalloproteases/genetics/*immunology/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Proteasome Endopeptidase Complex/genetics/immunology
MH  - Vaccinia/genetics/*immunology
MH  - Vaccinia virus/genetics/*immunology
PMC - PMC3323003
EDAT- 2012/02/03 06:00
MHDA- 2012/05/17 06:00
PMCR- 2013/03/23
CRDT- 2012/02/03 06:00
PHST- 2012/02/03 06:00 [entrez]
PHST- 2012/02/03 06:00 [pubmed]
PHST- 2012/05/17 06:00 [medline]
PHST- 2013/03/23 00:00 [pmc-release]
AID - S0021-9258(20)65412-6 [pii]
AID - M111.314856 [pii]
AID - 10.1074/jbc.M111.314856 [doi]
PST - ppublish
SO  - J Biol Chem. 2012 Mar 23;287(13):9990-10000. doi: 10.1074/jbc.M111.314856. Epub 
      2012 Feb 1.