PMID- 22299633
OWN - NLM
STAT- MEDLINE
DCOM- 20121011
LR  - 20171116
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 36
IP  - 7
DP  - 2012 Jul
TI  - Extracellular nucleotide inhibits cell proliferation and negatively regulates 
      Toll-like receptor 4 signalling in human progenitor endothelial cells.
PG  - 625-33
LID - 10.1042/CBI20110111 [doi]
AB  - Extracellular nucleotides mediate a wide range of physiological effects by 
      interacting with plasma membrane P2 purinergic receptors. P2 receptors are 
      expressed in certain kinds of stem cells, and function to induce cytokine 
      expression and to modulate cell proliferation. We have analysed the expression 
      and the function of P2 receptors in human umbilical cord blood-derived EPCs 
      (endothelial progenitor cells). EPCs expressed P2X4,6,7 and P2Y2,4,11,13,14 
      receptors and extracellular ATP inhibited EPCs proliferation. As in a previous 
      study, EPCs expressed functional TLR4 (Toll-like receptor 4) and activation of 
      TLR4 by LPS (lipopolysaccharide) evoked a pro-inflammatory immune response. When 
      human EPCs were stimulated with LPS and nucleotides, ATP or UTP inhibited the 
      expression of pro-inflammatory cytokines including MCP-1 (monocyte 
      chemoattractant protein-1), IFNalpha (interferon alpha), TNFalpha (tumour necrosis factor alpha) 
      and adhesion molecule VCAM-1 (vascular cell adhesion molecule 1) induced by LPS. 
      ATP and UTP also down-regulated the gene expression of TLR4, CD14 and MyD88 
      (myeloid differentiation factor 88), a TLR adaptor molecule, and protein 
      expression of CD14 and MyD88. Moreover, the phosphorylation of NF-kappaB (nuclear 
      factor kappaB) p65 induced by TLR4 activation was inhibited partly by ATP or UTP at 
      concentrations of 1-5 muM. These results suggest that extracellular nucleotides 
      negatively regulate EPCs proliferation and TLR4 signalling.
FAU - Xiao, Zhilin
AU  - Xiao Z
AD  - *Department of Geriatric Cardiology, Xiangya Hospital, Central South University, 
      Changsha 410008, People's Republic of China.
FAU - Yang, Mei
AU  - Yang M
FAU - Fang, Li
AU  - Fang L
FAU - Lv, Qingshan
AU  - Lv Q
FAU - He, Qing
AU  - He Q
FAU - Deng, Minjie
AU  - Deng M
FAU - Liu, Xueting
AU  - Liu X
FAU - Chen, Xiaobin
AU  - Chen X
FAU - Chen, Meifang
AU  - Chen M
FAU - Xie, Xiumei
AU  - Xie X
FAU - Hu, Jinyue
AU  - Hu J
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Receptors, Purinergic P2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Transcription Factor RelA)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - UT0S826Z60 (Uridine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/*pharmacology
MH  - Cell Proliferation/drug effects
MH  - Cytokines/genetics/metabolism
MH  - Down-Regulation
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Lipopolysaccharide Receptors/metabolism
MH  - Lipopolysaccharides/toxicity
MH  - Myeloid Differentiation Factor 88/metabolism
MH  - Phosphorylation
MH  - Receptors, Purinergic P2/metabolism
MH  - Signal Transduction/drug effects
MH  - Stem Cells/cytology/*metabolism
MH  - Toll-Like Receptor 4/genetics/*metabolism
MH  - Transcription Factor RelA/metabolism
MH  - Umbilical Cord/cytology
MH  - Uridine Triphosphate/*pharmacology
EDAT- 2012/02/04 06:00
MHDA- 2012/10/12 06:00
CRDT- 2012/02/04 06:00
PHST- 2012/02/04 06:00 [entrez]
PHST- 2012/02/04 06:00 [pubmed]
PHST- 2012/10/12 06:00 [medline]
AID - CBI20110111 [pii]
AID - 10.1042/CBI20110111 [doi]
PST - ppublish
SO  - Cell Biol Int. 2012 Jul;36(7):625-33. doi: 10.1042/CBI20110111.