PMID- 22300722
OWN - NLM
STAT- MEDLINE
DCOM- 20120622
LR  - 20220316
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 30
IP  - 12
DP  - 2012 Mar 9
TI  - Immunogenicity study of plant-made oral subunit vaccine against porcine 
      reproductive and respiratory syndrome virus (PRRSV).
PG  - 2068-74
LID - 10.1016/j.vaccine.2012.01.059 [doi]
AB  - Currently, killed-virus and modified-live PRRSV vaccines are used to control 
      porcine reproductive and respiratory syndrome disease (PRRS). However, very 
      limited efficacy of killed-virus vaccines and serious safety concerns for 
      modified-live virus vaccines demand the development of novel PRRSV vaccines. In 
      this report, we investigated the possibility of using transgenic plants as a 
      cost-effective and scalable system for production and delivery of a viral protein 
      as an oral subunit vaccine against PRRSV. Corn calli were genetically engineered 
      to produce PRRSV viral envelope-associated M protein. Both serum and intestine 
      mucosal antigen-specific antibodies were induced by oral administration of the 
      transgenic plant tissues to mice. In addition, serum and mucosal antibodies 
      showed virus neutralization activity. The neutralization antibody titers after 
      the final boost reached 6.7 in serum and 3.7 in fecal extracts, respectively. A 
      PRRSV-specific IFN-gamma response was also detected in splenocytes of vaccinated 
      animals. These results demonstrate that transgenic corn plants are an efficient 
      subunit vaccine production and oral delivery system for generation of both 
      systemic and mucosal immune responses against PRRSV.
CI  - Copyright A(c) 2012 Elsevier Ltd. All rights reserved.
FAU - Hu, Jianzhong
AU  - Hu J
AD  - Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA 
      24061, USA.
FAU - Ni, Yanyan
AU  - Ni Y
FAU - Dryman, Barbara A
AU  - Dryman BA
FAU - Meng, X J
AU  - Meng XJ
FAU - Zhang, Chenming
AU  - Zhang C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120131
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (M protein, porcine reproductive and respiratory syndrome virus)
RN  - 0 (Vaccines, Edible)
RN  - 0 (Vaccines, Subunit)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (Viral Vaccines)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antibodies, Neutralizing/analysis/blood
MH  - Antibodies, Viral/analysis/blood
MH  - Feces/chemistry
MH  - Female
MH  - Interferon-gamma/metabolism
MH  - Leukocytes, Mononuclear/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mucous Membrane/immunology
MH  - Plants, Genetically Modified/*genetics
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Spleen/immunology
MH  - Vaccines, Edible/administration & dosage/genetics/immunology
MH  - Vaccines, Subunit/administration & dosage/genetics/immunology
MH  - Vaccines, Synthetic/administration & dosage/genetics/immunology
MH  - Viral Matrix Proteins/genetics/*immunology
MH  - Viral Vaccines/*administration & dosage/genetics/*immunology
MH  - Zea mays/genetics
EDAT- 2012/02/04 06:00
MHDA- 2012/06/23 06:00
CRDT- 2012/02/04 06:00
PHST- 2011/12/01 00:00 [received]
PHST- 2012/01/14 00:00 [revised]
PHST- 2012/01/19 00:00 [accepted]
PHST- 2012/02/04 06:00 [entrez]
PHST- 2012/02/04 06:00 [pubmed]
PHST- 2012/06/23 06:00 [medline]
AID - S0264-410X(12)00097-7 [pii]
AID - 10.1016/j.vaccine.2012.01.059 [doi]
PST - ppublish
SO  - Vaccine. 2012 Mar 9;30(12):2068-74. doi: 10.1016/j.vaccine.2012.01.059. Epub 2012 
      Jan 31.