PMID- 22301695
OWN - NLM
STAT- MEDLINE
DCOM- 20120724
LR  - 20211021
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 19
IP  - 4
DP  - 2012 Apr
TI  - Serological diagnosis of paracoccidioidomycosis through a Western blot technique.
PG  - 616-9
LID - 10.1128/CVI.05693-11 [doi]
AB  - Paracoccidioidomycosis (PCM) is a serious infectious disease that progresses 
      toward death if untreated. Its confirmatory diagnosis is made by the detection of 
      the fungus Paracoccidioides brasiliensis in a direct mycological examination or 
      by histopathology. However, these techniques are of low sensitivity. Serological 
      tests seem to be more promising. The objective of this study was to test Western 
      blot (WB) analysis using sera from patients suspected of PCM to determine whether 
      it represents a safe and sensitive serological technique for a rapid and 
      effective diagnosis for this disease. Sera from 517 patients were analyzed 
      through WB analysis and double-immunodiffusion (DID) techniques using a crude 
      exoantigen of P. brasiliensis 339. DID gave positive reactions for 140 sera (27%) 
      and WB for 250 sera (48.4%). All sera that had a positive reaction by DID also 
      had a positive result with a 43-kDa glycoprotein by WB analysis. Among the 377 
      samples that were negative by DID, 29.1% were reactive in WB analysis. For the 
      cutoff dilution used (1:400), a positive reaction was not observed with any of 
      the 102 sera from patients with other diseases in regions where such diseases are 
      endemic and 30 healthy individuals tested as negative controls. These results 
      prove WB analysis to be a sensitive technique and suggest its inclusion among 
      routine laboratory assays as a safe method for PCM diagnosis.
FAU - Perenha-Viana, M C Z
AU  - Perenha-Viana MC
AD  - Departamento de Analises Clinicas e Biomedicina, Programa de Pos-graduacao em 
      Biociencias Aplicadas a Farmacia, Universidade Estadual de Maringa, Maringa, 
      Brazil.
FAU - Gonzales, I A A
AU  - Gonzales IA
FAU - Brockelt, S R
AU  - Brockelt SR
FAU - Machado, L N C
AU  - Machado LN
FAU - Svidzinski, T I E
AU  - Svidzinski TI
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120201
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antibodies, Fungal)
SB  - IM
MH  - Antibodies, Fungal/*blood
MH  - Blotting, Western/*methods
MH  - Humans
MH  - Mycology/*methods
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/*diagnosis
MH  - Sensitivity and Specificity
MH  - Serologic Tests/methods
MH  - Time Factors
PMC - PMC3318271
EDAT- 2012/02/04 06:00
MHDA- 2012/07/25 06:00
PMCR- 2012/10/01
CRDT- 2012/02/04 06:00
PHST- 2012/02/04 06:00 [entrez]
PHST- 2012/02/04 06:00 [pubmed]
PHST- 2012/07/25 06:00 [medline]
PHST- 2012/10/01 00:00 [pmc-release]
AID - CVI.05693-11 [pii]
AID - 05693-11 [pii]
AID - 10.1128/CVI.05693-11 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2012 Apr;19(4):616-9. doi: 10.1128/CVI.05693-11. Epub 2012 
      Feb 1.