PMID- 2230287
OWN - NLM
STAT- MEDLINE
DCOM- 19901205
LR  - 20151119
IS  - 0737-1454 (Print)
IS  - 0737-1454 (Linking)
VI  - 8
IP  - 5
DP  - 1990 Sep
TI  - The effect of carbamylcholine on CFU-s differentiation.
PG  - 377-84
AB  - The proportion of spleen colony-forming units (CFU-s) killed by hydroxyurea was 
      greatly increased after bone marrow cells (BMCs) from LACA mice were exposed to 
      carbamylcholine (Cach; 1 X 10(-13) to 1 X 10(-9) in vitro and there was a marked 
      change in the proportion of spleen colony types. Following treatment with Cach, 
      granulocytic and mixed erythroid-type colonies increased from 20 to 26.3% and 
      16.1 to 29.6% in 9-day colonies and from 8.3 to 28.2% and 21.7 to 39.4% in 13-day 
      colonies, respectively. Single cell suspensions of spleen colonies were made for 
      granulocyte-macrophage progenitor (CFU-gm) and late erythroid progenitor (CFU-e) 
      assays. The number of CFU-gm from Cach-treated BMC was about twice that from 
      control BMC for both day 9 and day 13 groups; the number of CFU-e decreased 
      relatively. The results suggest that cholinergic receptors on CFU-s may increase 
      the tendency to differentiate into the granulocytic/monocytic line.
FAU - Hu, X T
AU  - Hu XT
AD  - Research Laboratory of Blood Physiology, Hunan Medical University, Changsha, 
      People's Republic of China.
FAU - Xu, Y H
AU  - Xu YH
FAU - Zhou, Y J
AU  - Zhou YJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cell Cloning
JT  - International journal of cell cloning
JID - 8308172
RN  - 8Y164V895Y (Carbachol)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - Carbachol/*pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cell Division/drug effects
MH  - Colony-Forming Units Assay
MH  - Female
MH  - Hematopoietic Stem Cells/cytology/*drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Spleen/cytology
MH  - Whole-Body Irradiation
EDAT- 1990/09/01 00:00
MHDA- 1990/09/01 00:01
CRDT- 1990/09/01 00:00
PHST- 1990/09/01 00:00 [pubmed]
PHST- 1990/09/01 00:01 [medline]
PHST- 1990/09/01 00:00 [entrez]
AID - 10.1002/stem.5530080506 [doi]
PST - ppublish
SO  - Int J Cell Cloning. 1990 Sep;8(5):377-84. doi: 10.1002/stem.5530080506.