PMID- 22303754
OWN - NLM
STAT- MEDLINE
DCOM- 20120223
LR  - 20220318
IS  - 1538-8506 (Print)
IS  - 1538-8506 (Linking)
VI  - 24
IP  - 4
DP  - 2011 Dec
TI  - Using animal models in osteoarthritis biomarker research.
PG  - 251-64
AB  - Osteoarthritis (OA) is a disease that commonly affects human and veterinary 
      patients. Animal models are routinely used for OA research, and the dog is a 
      nearly ideal species for translational investigation of human OA biomarkers. The 
      cytokine, chemokine, and matrix metalloprotease (MMP) profiles of synovial fluid, 
      serum, and urine from dogs with surgically induced and naturally occurring OA 
      were compared with dogs without OA using xMAP technology (Qiagen Inc., Valencia, 
      CA). Markers that exhibited significant differences between groups were 
      identified (monocyte chemoattractant protein 1 [MCP1], interleukin 8 [IL8], 
      keratinocyte-derived chemoattractant [KC], and MMP2 and MMP3), and their 
      sensitivities and specificities were calculated to determine their diagnostic 
      usefulness in a future biomarker panel. Synovial fluid IL8 was the most 
      sensitive, but MCP1 was also highly sensitive and specific. The alterations in KC 
      suggested that it may differentiate between cruciate disease and other types of 
      OA, and the MMPs were most sensitive and specific in the serum. This study 
      provided additional insight to the participation of cytokines, chemokines, and 
      MMPs in OA, and potential diagnostic biomarker candidates were identified. A 
      brief literature review of other biomarker candidates previously examined using 
      animal models is discussed.
FAU - Garner, Bridget C
AU  - Garner BC
AD  - Department of Veterinary Pathology, University of Georgia, Athens, GA 30605, USA. 
      garnerb@uga.edu
FAU - Stoker, Aaron M
AU  - Stoker AM
FAU - Kuroki, Keiichi
AU  - Kuroki K
FAU - Evans, Richard
AU  - Evans R
FAU - Cook, Cristi Reeves
AU  - Cook CR
FAU - Cook, James L
AU  - Cook JL
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Germany
TA  - J Knee Surg
JT  - The journal of knee surgery
JID - 101137599
RN  - 0 (Biomarkers)
RN  - 0 (Cartilage Oligomeric Matrix Protein)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Interleukin-8)
RN  - 0 (Matrilin Proteins)
RN  - 0 (TSP5 protein, human)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Arthroscopy
MH  - Biomarkers/*metabolism
MH  - Cartilage Oligomeric Matrix Protein
MH  - Chemokine CCL2
MH  - Chemokines/metabolism
MH  - Cytokines/*metabolism/urine
MH  - *Disease Models, Animal
MH  - Dogs
MH  - Extracellular Matrix Proteins/metabolism
MH  - Female
MH  - Glycoproteins/metabolism
MH  - Interleukin-8/metabolism
MH  - Matrilin Proteins
MH  - Matrix Metalloproteinases/metabolism
MH  - Osteoarthritis/*metabolism/physiopathology
MH  - Sensitivity and Specificity
MH  - Synovial Fluid/chemistry/metabolism
EDAT- 2012/02/07 06:00
MHDA- 2012/02/24 06:00
CRDT- 2012/02/07 06:00
PHST- 2012/02/07 06:00 [entrez]
PHST- 2012/02/07 06:00 [pubmed]
PHST- 2012/02/24 06:00 [medline]
AID - 10.1055/s-0031-1297361 [doi]
PST - ppublish
SO  - J Knee Surg. 2011 Dec;24(4):251-64. doi: 10.1055/s-0031-1297361.