PMID- 22304363
OWN - NLM
STAT- MEDLINE
DCOM- 20130503
LR  - 20121108
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 47
IP  - 6
DP  - 2012 Dec
TI  - Early cleaved bovine embryos show reduced incidence of chromosomal aberrations 
      and higher developmental potential on day 4.5 post-insemination.
PG  - 899-906
LID - 10.1111/j.1439-0531.2012.01987.x [doi]
AB  - The present study aimed to investigate whether the timing of the first zygotic 
      cleavage (FZC) influences the speed of embryo development expressed by the total 
      cell count and the rate of chromosomally aberrant embryos. Bovine embryos were 
      produced in vitro and divided into two categories according to the timing of FZC: 
      early cleavers (at 30 hpi; EC) and non-early cleavers (at 48 hpi; NEC). On day 
      4.5 pi, embryos were grouped into three classes depending on the number of 
      blastomeres: delayed (<8 BL), normal (8-16 BL) and advanced (>16 BL). We applied 
      fluorescence in situ hybridization (FISH) with probes for bovine chromosomes 6 
      and X. The only form of chromosomal imbalance observed was mixoploidy [(2n/3n; 
      2n/4n); 19.9%, 54/271]. Early cleavers were less often chromosomally unbalanced 
      (13.9%, 20/144) than their NEC counterparts (26.7%, 34/127). Among embryos 
      developing at a normal speed, the NEC embryos were more often abnormal (NEC 
      20/80; EC 10/79; p < 0.05). The advanced embryos were not observed among the NEC 
      category, whereas such embryos from EC category displayed no chromosomal 
      aberrations. The majority of embryos arrested at the 8 BL stage were of NEC 
      category and were carriers of chromosomally abnormal blastomeres. With regard to 
      embryonic sex, we demonstrated that although males dominate among bovine embryos 
      developing in vitro, the incidence of mixoploidy was equal for both sexes. It can 
      be suggested that a good-quality bovine embryo is usually an early cleaver that 
      develops at higher speed and contains less aberrant cells. The present study also 
      confirmed the usefulness of the FZC as a marker of embryo quality by 
      demonstrating a significantly lower incidence of aberrations in early embryos.
CI  - (c) 2012 Blackwell Verlag GmbH.
FAU - Pers-Kamczyc, E
AU  - Pers-Kamczyc E
AD  - Department of Genetics and Animal Breeding, Poznan University of Life Sciences, 
      Poznan, Poland.
FAU - Pawlak, P
AU  - Pawlak P
FAU - Rubes, J
AU  - Rubes J
FAU - Lechniak, D
AU  - Lechniak D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120204
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
SB  - IM
MH  - Animals
MH  - Cattle
MH  - *Chromosome Aberrations
MH  - Embryo Culture Techniques
MH  - *Embryonic Development
MH  - Female
MH  - Fertilization in Vitro/veterinary
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - Male
EDAT- 2012/02/07 06:00
MHDA- 2013/05/04 06:00
CRDT- 2012/02/07 06:00
PHST- 2012/02/07 06:00 [entrez]
PHST- 2012/02/07 06:00 [pubmed]
PHST- 2013/05/04 06:00 [medline]
AID - 10.1111/j.1439-0531.2012.01987.x [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2012 Dec;47(6):899-906. doi: 
      10.1111/j.1439-0531.2012.01987.x. Epub 2012 Feb 4.