PMID- 22304777
OWN - NLM
STAT- MEDLINE
DCOM- 20130109
LR  - 20191210
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 28
IP  - 2
DP  - 2012 Feb
TI  - [Effect of IL-17 on the expression of monocyte chemoattractant protein-1 in the 
      cardiac myocytes].
PG  - 163-6
AB  - AIM: To investigate the effect of interleukin-17 (IL-17) on the expression of 
      monocyte chemoattractant protein-1(MCP-1) in the primary cultured cardiac 
      myocytes. METHODS: The cardiac myocytes were isolated from neonatal mice by 
      different adhesion method. The expressions of IL-17R and MCP-1 in the cardiac 
      myocytes were evaluated by reverse transcription-polymerase chain reaction 
      (RT-PCR). And the concentration of MCP-1 in the culture supernatant of the 
      cardiac myocytes was detected by enzyme-linked immunosorbent assay (ELISA). 
      RESULTS: There was the expression of IL-17R in the cardiac myocytes. After 
      stimulated by IL-17, the expressions of MCP-1 in the cardiac myocytes were 
      significantly increased in dose-dependent manner compared with that of culture 
      medium control (P<0.05). The amount of MCP-1 mRNA in the cardiac myocytes was the 
      highest at 4 h after stimulated by IL-17. And then the amount of MCP-1 mRNA in 
      the cardiac myocytes began to descend. The concentrations of MCP-1 in the culture 
      supernatant of the cardiac myocytes increased in time-dependent manner and had 
      significant differences with the control group (P<0.05). CONCLUSION: The cardiac 
      myocytes could express IL-17R. IL-17 could up-regulate the expression of MCP-1 in 
      the cardiac myocytes, which was associated with the dose and stimulation time of 
      IL-17.
FAU - Liu, Yan-yue
AU  - Liu YY
AD  - Department of Clinical Laboratory Medicine, The First Affiliated Hospital of 
      Zhengzhou University, Zhengzhou, China. liuyueyan2005@163.com
FAU - Li, Yan-yan
AU  - Li YY
FAU - Wang, Shan-shan
AU  - Wang SS
FAU - Shen, Yan
AU  - Shen Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Chemokine CCL2)
RN  - 0 (Il17ra protein, mouse)
RN  - 0 (Interleukin-17)
RN  - 0 (Receptors, Interleukin-17)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*genetics/metabolism
MH  - Gene Expression Regulation/*drug effects
MH  - Interleukin-17/*pharmacology
MH  - Mice
MH  - Myocytes, Cardiac/*drug effects/*metabolism
MH  - Primary Cell Culture
MH  - Receptors, Interleukin-17/genetics
EDAT- 2012/02/07 06:00
MHDA- 2013/01/10 06:00
CRDT- 2012/02/07 06:00
PHST- 2012/02/07 06:00 [entrez]
PHST- 2012/02/07 06:00 [pubmed]
PHST- 2013/01/10 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Feb;28(2):163-6.