PMID- 22306009
OWN - NLM
STAT- MEDLINE
DCOM- 20120507
LR  - 20211021
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 419
IP  - 2
DP  - 2012 Mar 9
TI  - Biophysical characterisation of the nucleocapsid protein from a highly pathogenic 
      porcine reproductive and respiratory syndrome virus strain.
PG  - 137-41
LID - 10.1016/j.bbrc.2011.11.126 [doi]
AB  - The arterivirus nucleocapsid (N) protein is a multifunctional protein that binds 
      viral RNA for encapsidation and has potential roles in host cell processes. This 
      study characterised the N protein from a highly virulent North American strain of 
      porcine reproductive and respiratory syndrome virus (PRRSV). The association with 
      viral RNA was mapped to defined motifs on the N protein. The results indicated 
      that disulphide bridge formation played a key role in RNA binding, offering an 
      explanation why infectious virus cannot be rescued if cysteine residues are 
      mutated, and that multiple sites may promote RNA binding.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Jourdan, Stefanie S
AU  - Jourdan SS
AD  - Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, 
      Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 
      6JP, UK.
FAU - Osorio, Fernando A
AU  - Osorio FA
FAU - Hiscox, Julian A
AU  - Hiscox JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120128
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (RNA, Viral)
RN  - K848JZ4886 (Cysteine)
RN  - OF5P57N2ZX (Alanine)
SB  - IM
MH  - Alanine/genetics/metabolism
MH  - Amino Acid Substitution
MH  - Animals
MH  - Cysteine/genetics/*metabolism
MH  - Nucleocapsid Proteins/genetics/*metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*pathogenicity
MH  - RNA, Viral/*metabolism
MH  - Swine
PMC - PMC7092862
EDAT- 2012/02/07 06:00
MHDA- 2012/05/09 06:00
PMCR- 2012/01/28
CRDT- 2012/02/07 06:00
PHST- 2011/11/11 00:00 [received]
PHST- 2011/11/29 00:00 [accepted]
PHST- 2012/02/07 06:00 [entrez]
PHST- 2012/02/07 06:00 [pubmed]
PHST- 2012/05/09 06:00 [medline]
PHST- 2012/01/28 00:00 [pmc-release]
AID - S0006-291X(11)02154-1 [pii]
AID - 10.1016/j.bbrc.2011.11.126 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 Mar 9;419(2):137-41. doi: 
      10.1016/j.bbrc.2011.11.126. Epub 2012 Jan 28.