PMID- 22306699
OWN - NLM
STAT- MEDLINE
DCOM- 20120924
LR  - 20220316
IS  - 1873-3441 (Electronic)
IS  - 0939-6411 (Linking)
VI  - 81
IP  - 1
DP  - 2012 May
TI  - Efficacy of thiolated eudragit microspheres as an oral vaccine delivery system to 
      induce mucosal immunity against enterotoxigenic Escherichia coli in mice.
PG  - 43-8
LID - 10.1016/j.ejpb.2012.01.010 [doi]
AB  - A vaccine delivery system based on thiolated eudragit microsphere (TEMS) was 
      studied in vivo for its ability to elicit mucosal immunity against 
      enterotoxigenic Escherichia coli (ETEC). Groups of mice were orally immunized 
      with F4 or F18 fimbriae of ETEC and F4 or F18 loaded in TEMS. Mice that were 
      orally administered with F4 or F18 loaded TEMS showed higher antigen-specific IgG 
      antibody responses in serum and antigen-specific IgA in saliva and feces than 
      mice that were immunized with antigens only. In addition, oral vaccination of F4 
      or F18 loaded TEMS resulted in higher numbers of IgG and IgA antigen-specific 
      antibody secreting cells in the spleen, lamina propria, and Peyer's patches of 
      immunized mice than other groups. Moreover, TEMS administration loaded with F4 or 
      F18 induced mixed Th1 and Th2 type responses based on similarly increased levels 
      of IgG1 and IgG2a. These results suggest that F4 or F18 loaded TEMS may be a 
      promising candidate for an oral vaccine delivery system to elicit systemic and 
      mucosal immunity against ETEC.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Lee, Won-Jung
AU  - Lee WJ
AD  - Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic 
      Disease Priority Research Institute, Seoul, Republic of Korea.
FAU - Cha, Seungbin
AU  - Cha S
FAU - Shin, Minkyoung
AU  - Shin M
FAU - Jung, Myunghwan
AU  - Jung M
FAU - Islam, Mohammad Ariful
AU  - Islam MA
FAU - Cho, Chong-su
AU  - Cho CS
FAU - Yoo, Han Sang
AU  - Yoo HS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120128
PL  - Netherlands
TA  - Eur J Pharm Biopharm
JT  - European journal of pharmaceutics and biopharmaceutics : official journal of 
      Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
JID - 9109778
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (FedA protein, E coli)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (K88 antigen, E coli)
RN  - 0 (Polymethacrylic Acids)
RN  - 0 (Sulfhydryl Compounds)
RN  - 0 (Vaccines)
RN  - 147680-16-8 (Fimbriae Proteins)
RN  - 25086-15-1 (methylmethacrylate-methacrylic acid copolymer)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antigens, Bacterial/administration & dosage/immunology
MH  - Enterotoxigenic Escherichia coli/*immunology
MH  - Escherichia coli Infections/*immunology
MH  - Escherichia coli Proteins/administration & dosage/immunology
MH  - Female
MH  - Fimbriae Proteins/administration & dosage/immunology
MH  - Immunity, Mucosal
MH  - Immunoglobulin A/immunology
MH  - Immunoglobulin G/immunology
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Microspheres
MH  - Polymethacrylic Acids/*chemistry
MH  - Sulfhydryl Compounds/chemistry
MH  - Vaccines/administration & dosage/*immunology
EDAT- 2012/02/07 06:00
MHDA- 2012/09/25 06:00
CRDT- 2012/02/07 06:00
PHST- 2011/08/01 00:00 [received]
PHST- 2011/12/30 00:00 [revised]
PHST- 2012/01/18 00:00 [accepted]
PHST- 2012/02/07 06:00 [entrez]
PHST- 2012/02/07 06:00 [pubmed]
PHST- 2012/09/25 06:00 [medline]
AID - S0939-6411(12)00011-2 [pii]
AID - 10.1016/j.ejpb.2012.01.010 [doi]
PST - ppublish
SO  - Eur J Pharm Biopharm. 2012 May;81(1):43-8. doi: 10.1016/j.ejpb.2012.01.010. Epub 
      2012 Jan 28.