PMID- 22307293
OWN - NLM
STAT- MEDLINE
DCOM- 20120709
LR  - 20211021
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 78
IP  - 8
DP  - 2012 Apr
TI  - Diversity of symbiotic organs and bacterial endosymbionts of lygaeoid bugs of the 
      families blissidae and lygaeidae (hemiptera: heteroptera: lygaeoidea).
PG  - 2648-59
LID - 10.1128/AEM.07191-11 [doi]
AB  - Here we present comparative data on the localization and identity of 
      intracellular symbionts among the superfamily Lygaeoidea (Insecta: Hemiptera: 
      Heteroptera: Pentatomomorpha). Five different lygaeoid species from the families 
      Blissidae and Lygaeidae (sensu stricto; including the subfamilies Lygaeinae and 
      Orsillinae) were analyzed. Fluorescence in situ hybridization (FISH) revealed 
      that all the bugs studied possess paired bacteriomes that are differently shaped 
      in the abdomen and harbor specific endosymbionts therein. The endosymbionts were 
      also detected in female gonads and at the anterior poles of developing eggs, 
      indicating vertical transmission of the endosymbionts via ovarial passage, in 
      contrast to the posthatch symbiont transmission commonly found among pentatomoid 
      bugs (Pentatomomorpha: Pentatomoidea). Phylogenetic analysis based on 16S rRNA 
      and groEL genes showed that the endosymbionts of Ischnodemus sabuleti, Arocatus 
      longiceps, Belonochilus numenius, Orsillus depressus, and Ortholomus punctipennis 
      constitute at least four distinct clades in the Gammaproteobacteria. The 
      endosymbiont phylogeny did not agree with the host phylogeny based on the 
      mitochondrial cytochrome oxidase I (COI) gene, but there was a local cospeciating 
      pattern within the subfamily Orsillinae. Meanwhile, the endosymbiont of 
      Belonochilus numenius (Lygaeidae: Orsillinae), although harbored in paired 
      bacteriomes as in other lygaeoid bugs of the related genera Nysius, Ortholomus, 
      and Orsillus, was phylogenetically close to "Candidatus Rohrkolberia cinguli," 
      the endosymbiont of Chilacis typhae (Lygaeoidea: Artheneidae), suggesting an 
      endosymbiont replacement in this lineage. The diverse endosymbionts and the 
      differently shaped bacteriomes may reflect independent evolutionary origins of 
      the endosymbiotic systems among lygaeoid bugs.
FAU - Kuechler, Stefan Martin
AU  - Kuechler SM
AD  - Department of Animal Ecology II, University of Bayreuth, Bayreuth, Germany. 
      stefan.kuechler@uni-bayreuth.de
FAU - Renz, Patricia
AU  - Renz P
FAU - Dettner, Konrad
AU  - Dettner K
FAU - Kehl, Siegfried
AU  - Kehl S
LA  - eng
SI  - GENBANK/HE586112
SI  - GENBANK/HE586113
SI  - GENBANK/HE586114
SI  - GENBANK/HE586115
SI  - GENBANK/HE586116
SI  - GENBANK/HE586117
SI  - GENBANK/HE586258
SI  - GENBANK/HE586259
SI  - GENBANK/HE586260
SI  - GENBANK/HE586261
SI  - GENBANK/HE586262
SI  - GENBANK/HE586263
SI  - GENBANK/HE586264
SI  - GENBANK/HE586265
SI  - GENBANK/HE586266
SI  - GENBANK/HE586267
SI  - GENBANK/HE586268
SI  - GENBANK/HE586269
PT  - Journal Article
DEP - 20120203
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Chaperonin 60)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animal Structures/*microbiology
MH  - Animals
MH  - *Bacterial Physiological Phenomena
MH  - Chaperonin 60/genetics
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Gammaproteobacteria/classification/genetics/*isolation & purification
MH  - Heteroptera/*microbiology/physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - *Symbiosis
PMC - PMC3318820
EDAT- 2012/02/07 06:00
MHDA- 2012/07/10 06:00
PMCR- 2012/10/01
CRDT- 2012/02/07 06:00
PHST- 2012/02/07 06:00 [entrez]
PHST- 2012/02/07 06:00 [pubmed]
PHST- 2012/07/10 06:00 [medline]
PHST- 2012/10/01 00:00 [pmc-release]
AID - AEM.07191-11 [pii]
AID - 07191-11 [pii]
AID - 10.1128/AEM.07191-11 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2012 Apr;78(8):2648-59. doi: 10.1128/AEM.07191-11. Epub 
      2012 Feb 3.