PMID- 22307334
OWN - NLM
STAT- MEDLINE
DCOM- 20120612
LR  - 20171206
IS  - 1724-6040 (Electronic)
IS  - 0391-3988 (Linking)
VI  - 35
IP  - 1
DP  - 2012 Jan
TI  - Characterization and chondrogenic differentiation of menstrual blood-derived stem 
      cells on a nanofibrous scaffold.
PG  - 55-66
LID - 10.5301/ijao.5000019 [doi]
AB  - INTRODUCTION: The recent identification of menstrual blood-derived stem cells 
      (MenSCs) as a unique population of stem cells has created enormous promise for 
      tissue engineering. In this study, after characterization of MenSCs in comparison 
      with bone marrow-derived stem cells (BMSCs), the potential of MenSCs seeded into 
      electrospun, biodegradable, nanofibrous scaffolds in order to engineer cartilage 
      was evaluated. METHODS: MenSCs and BMSCs were isolated by discontinuous density 
      gradient centrifugation and plastic adherence. After characterization of MenSCs 
      compared with BMSCs, MenSC differentiation into chondrocytes was investigated on 
      a nanofibrous scaffold with specific growth and differentiation factors. The 
      scaffold was prepared from polycaprolactone (PCL) and its surface was modified by 
      plasma treatment. RESULTS: Flow cytometric analysis of expanded cells showed that 
      MenSCs typically express some surface and intracellular markers associated with 
      BMSCs. But marked expression of OCT-4 and the absence of STRO1 distinguished them 
      from mesenchymal stem cells obtained from bone marrow. Based on scanning electron 
      microscope images, the MenSCs were strongly anchored to the highly porous 
      scaffold, which they penetrated and proliferated on. The scaffold contained an 
      extensive cartilage-like extracellular matrix with about 50% greater 
      glycosaminoglycan content than control MenSCs differentiated in a two-dimensional 
      (2D) culture system (p<0.05). Considerable amounts of proteoglycan were produced 
      by the cells differentiated on the scaffold, as demonstrated by Alcian blue 
      staining. Unlike undifferentiated MenSCs, cells differentiated on the scaffold 
      had strong immunoreactivity with monoclonal antibody against collagen type II. 
      CONCLUSIONS: The evidence presented in this study introduces MenSCs as a suitable 
      stem cell population candidate for cartilage tissue engineering.
FAU - Kazemnejad, Somaieh
AU  - Kazemnejad S
AD  - Department of Embryology and Stem Cells, Reproductive Biotechnology Research 
      Center, Avicenna Research Institute, ACECR, Tehran, Iran. kazemnejad_s@yahoo.com
FAU - Akhondi, Mohammad-Mehdi
AU  - Akhondi MM
FAU - Soleimani, Masoud
AU  - Soleimani M
FAU - Zarnani, Amir Hassan
AU  - Zarnani AH
FAU - Khanmohammadi, Manijeh
AU  - Khanmohammadi M
FAU - Darzi, Saeedeh
AU  - Darzi S
FAU - Alimoghadam, Kamran
AU  - Alimoghadam K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Artif Organs
JT  - The International journal of artificial organs
JID - 7802649
RN  - 0 (Biomarkers)
RN  - 0 (Collagen Type II)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Polyesters)
RN  - 0 (Proteoglycans)
RN  - 24980-41-4 (polycaprolactone)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/physiology
MH  - Cell Adhesion
MH  - Cell Culture Techniques
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Chondrocytes/immunology/metabolism/*physiology/ultrastructure
MH  - *Chondrogenesis
MH  - Collagen Type II/metabolism
MH  - Extracellular Matrix/metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Glycosaminoglycans/metabolism
MH  - Humans
MH  - Immunophenotyping
MH  - Menstruation/*blood
MH  - Microscopy, Electron, Scanning
MH  - *Nanofibers
MH  - Nanotechnology
MH  - Phenotype
MH  - Polyesters/*chemistry
MH  - Porosity
MH  - Proteoglycans/metabolism
MH  - Stem Cells/immunology/metabolism/*physiology/ultrastructure
MH  - Surface Properties
MH  - Time Factors
MH  - *Tissue Engineering
MH  - *Tissue Scaffolds
EDAT- 2012/02/07 06:00
MHDA- 2012/06/13 06:00
CRDT- 2012/02/07 06:00
PHST- 2011/07/15 00:00 [accepted]
PHST- 2012/02/07 06:00 [entrez]
PHST- 2012/02/07 06:00 [pubmed]
PHST- 2012/06/13 06:00 [medline]
AID - 5C53F593-7FED-4C78-B1ED-AC006537EC2B [pii]
AID - 10.5301/ijao.5000019 [doi]
PST - ppublish
SO  - Int J Artif Organs. 2012 Jan;35(1):55-66. doi: 10.5301/ijao.5000019.