PMID- 22308402
OWN - NLM
STAT- MEDLINE
DCOM- 20120330
LR  - 20220311
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 109
IP  - 6
DP  - 2012 Feb 7
TI  - Host RNAs, including transposons, are encapsidated by a eukaryotic 
      single-stranded RNA virus.
PG  - 1907-12
LID - 10.1073/pnas.1116168109 [doi]
AB  - Next-generation sequencing is a valuable tool in our growing understanding of the 
      genetic diversity of viral populations. Using this technology, we have 
      investigated the RNA content of a purified nonenveloped single-stranded RNA 
      virus, flock house virus (FHV). We have also investigated the RNA content of 
      virus-like particles (VLPs) of FHV and the related Nudaurelia capensis omega 
      virus. VLPs predominantly package ribosomal RNA and transcripts of their 
      baculoviral expression vectors. In addition, we find that 5.3% of the packaged 
      RNAs are transposable elements derived from the Sf21 genome. This observation may 
      be important when considering the therapeutic use of VLPs. We find that authentic 
      FHV virions also package a variety of host RNAs, accounting for 1% of the 
      packaged nucleic acid. Significant quantities of host messenger RNAs, ribosomal 
      RNA, noncoding RNAs, and transposable elements are readily detected. The 
      packaging of these host RNAs elicits the possibility of horizontal gene transfer 
      between eukaryotic hosts that share a viral pathogen. We conclude that the 
      genetic content of nonenveloped RNA viruses is variable, not just by genome 
      mutation, but also in the diversity of RNA transcripts that are packaged.
FAU - Routh, Andrew
AU  - Routh A
AD  - Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 
      92037, USA.
FAU - Domitrovic, Tatiana
AU  - Domitrovic T
FAU - Johnson, John E
AU  - Johnson JE
LA  - eng
GR  - R37 GM034220/GM/NIGMS NIH HHS/United States
GR  - R37-GM034220/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120124
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA Transposable Elements)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Capsid/*metabolism/ultrastructure
MH  - Cell Line
MH  - DNA Transposable Elements/*genetics
MH  - Eukaryota/*genetics
MH  - Gene Expression Regulation, Viral
MH  - Genome, Viral/genetics
MH  - Host-Pathogen Interactions/*genetics
MH  - Mutation Rate
MH  - RNA Viruses/*genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Viral/*genetics/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Analysis, RNA
MH  - Virion/ultrastructure
MH  - Virus Assembly/genetics
PMC - PMC3277583
COIS- The authors declare no conflict of interest.
EDAT- 2012/02/07 06:00
MHDA- 2012/03/31 06:00
PMCR- 2012/08/07
CRDT- 2012/02/07 06:00
PHST- 2012/02/07 06:00 [entrez]
PHST- 2012/02/07 06:00 [pubmed]
PHST- 2012/03/31 06:00 [medline]
PHST- 2012/08/07 00:00 [pmc-release]
AID - 1116168109 [pii]
AID - 201116168 [pii]
AID - 10.1073/pnas.1116168109 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2012 Feb 7;109(6):1907-12. doi: 
      10.1073/pnas.1116168109. Epub 2012 Jan 24.