PMID- 22309649
OWN - NLM
STAT- MEDLINE
DCOM- 20130103
LR  - 20181201
IS  - 1347-4421 (Electronic)
IS  - 1347-4421 (Linking)
VI  - 113
IP  - 6
DP  - 2012 Jun
TI  - Selective enumeration of viable Enterobacteriaceae and Pseudomonas spp. in milk 
      within 7 h by multicolor fluorescence in situ hybridization following microcolony 
      formation.
PG  - 746-50
LID - 10.1016/j.jbiosc.2012.01.009 [doi]
AB  - Rapid and simultaneous enumeration of viable Enterobacteriaceae and viable 
      Pseudomonas spp. in milk was achieved by using multicolor fluorescence in situ 
      hybridization (FISH) with oligonucleotide probes based on 16S ribosomal RNA 
      (rRNA) sequences in combination with a microcolony growth method (multicolor 
      microcolony-FISH; MMC-FISH). The procedure of MMC-FISH method is rather simple; 
      that is milk clearing, filtration of cells, incubation, hybridization and 
      enumeration. Enumeration of targeted bacteria in logarithmic growth phase, 
      stationary phase, or in a starved state in milk by MMC-FISH required 5-7 h, while 
      it took 1-3 days to test for Escherichia coli and Pseudomonas putida by the 
      conventional culture method. The numbers of E. coli and P. putida in each phase 
      or in a starved state in milk determined by MMC-FISH were almost the same or 
      greater than the number of colony forming units determined by the plate counting 
      method. The MMC-FISH allows rapid examination of contamination in milk by viable 
      Enterobacteriaceae and Pseudomonas spp. with growth potential.
CI  - Copyright (c) 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. 
      All rights reserved.
FAU - Yamaguchi, Nobuyasu
AU  - Yamaguchi N
AD  - Environmental Science and Microbiology, Graduate School of Pharmaceutical 
      Science, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan. 
      yamaguti@phs.osaka-u.ac.jp
FAU - Kitaguchi, Akiko
AU  - Kitaguchi A
FAU - Nasu, Masao
AU  - Nasu M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120205
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Colony Count, Microbial/*methods
MH  - Enterobacteriaceae/*genetics/growth & development
MH  - Escherichia coli/genetics/growth & development
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Milk/microbiology
MH  - Oligonucleotide Probes
MH  - Pseudomonas/*genetics/growth & development
MH  - RNA, Ribosomal, 16S
EDAT- 2012/02/09 06:00
MHDA- 2013/01/04 06:00
CRDT- 2012/02/08 06:00
PHST- 2011/11/17 00:00 [received]
PHST- 2012/01/11 00:00 [revised]
PHST- 2012/01/12 00:00 [accepted]
PHST- 2012/02/08 06:00 [entrez]
PHST- 2012/02/09 06:00 [pubmed]
PHST- 2013/01/04 06:00 [medline]
AID - S1389-1723(12)00030-8 [pii]
AID - 10.1016/j.jbiosc.2012.01.009 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 2012 Jun;113(6):746-50. doi: 10.1016/j.jbiosc.2012.01.009. Epub 
      2012 Feb 5.