PMID- 22310716
OWN - NLM
STAT- MEDLINE
DCOM- 20120416
LR  - 20181201
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 418
IP  - 4
DP  - 2012 Feb 24
TI  - NR4A nuclear receptors mediate carnitine palmitoyltransferase 1A gene expression 
      by the rexinoid HX600.
PG  - 780-5
LID - 10.1016/j.bbrc.2012.01.102 [doi]
AB  - Retinoid X receptors (RXRs) are members of the nuclear receptor superfamily and 
      can be activated by 9-cis retinoic acid (9CRA). RXRs form homodimers and 
      heterodimers with other nuclear receptors such as the retinoic acid receptor and 
      NR4 subfamily nuclear receptors, Nur77 and NURR1. Potential physiological roles 
      of the Nur77-RXR and NURR1-RXR heterodimers have not been elucidated. In this 
      study, we identified a gene regulated by these heterodimers utilizing HX600, a 
      selective RXR agonist for Nur77-RXR and NURR1-RXR. While 9CRA induced many genes, 
      including RAR-target genes, HX600 effectively induced only carnitine 
      palmitoyltransferase 1A (CPT1A) in human teratocarcinoma NT2/D1 cells, which 
      express RXRalpha, Nur77 and NURR1. HX600 also increased CPT1A expression in human 
      embryonic kidney (HEK) 293 cells and hepatocyte-derived HepG2 cells. Although 
      HX600 induced CPT1A less effectively than 9CRA, overexpression of Nur77 or NURR1 
      increased the HX600 response to levels similar to 9CRA in NT2/D1 and HEK293 
      cells. A dominant-negative form of Nur77 or NURR1 repressed the induction of 
      CPT1A by HX600. A protein synthesis inhibitor did not alter HX600-dependent CPT1A 
      induction. Thus, the rexinoid HX600 directly induces expression of CPT1A through 
      a Nur77 or NURR1-mediated mechanism. CPT1A, a gene involved in fatty acid 
      beta-oxidation, could be a target of RXR-NR4 receptor heterodimers.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Ishizawa, Michiyasu
AU  - Ishizawa M
AD  - Division of Biochemistry, Department of Biomedical Sciences, Nihon University 
      School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, Japan.
FAU - Kagechika, Hiroyuki
AU  - Kagechika H
FAU - Makishima, Makoto
AU  - Makishima M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120128
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Dibenzazepines)
RN  - 0 (NR4A1 protein, human)
RN  - 0 (NR4A2 protein, human)
RN  - 0 (Nuclear Receptor Subfamily 4, Group A, Member 1)
RN  - 0 (Nuclear Receptor Subfamily 4, Group A, Member 2)
RN  - 0 (Retinoid X Receptors)
RN  - 172705-89-4 (HX 600)
RN  - 1UA8E65KDZ (Alitretinoin)
RN  - 5688UTC01R (Tretinoin)
RN  - EC 2.3.1.21 (Carnitine O-Palmitoyltransferase)
SB  - IM
MH  - Alitretinoin
MH  - Animals
MH  - Carnitine O-Palmitoyltransferase
MH  - Cell Line, Tumor
MH  - Dibenzazepines/*pharmacology
MH  - Gene Expression/*drug effects
MH  - HEK293 Cells
MH  - Humans
MH  - Mice
MH  - Nuclear Receptor Subfamily 4, Group A, Member 1/genetics/*metabolism
MH  - Nuclear Receptor Subfamily 4, Group A, Member 2/genetics/*metabolism
MH  - Protein Multimerization
MH  - Rats
MH  - Retinoid X Receptors/metabolism
MH  - Tretinoin/pharmacology
EDAT- 2012/02/09 06:00
MHDA- 2012/04/17 06:00
CRDT- 2012/02/08 06:00
PHST- 2012/01/11 00:00 [received]
PHST- 2012/01/21 00:00 [accepted]
PHST- 2012/02/08 06:00 [entrez]
PHST- 2012/02/09 06:00 [pubmed]
PHST- 2012/04/17 06:00 [medline]
AID - S0006-291X(12)00149-0 [pii]
AID - 10.1016/j.bbrc.2012.01.102 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 Feb 24;418(4):780-5. doi: 
      10.1016/j.bbrc.2012.01.102. Epub 2012 Jan 28.