PMID- 22311844
OWN - NLM
STAT- MEDLINE
DCOM- 20120518
LR  - 20191210
IS  - 2042-6984 (Electronic)
IS  - 2042-6976 (Linking)
VI  - 2
IP  - 1
DP  - 2012 Jan-Feb
TI  - Mucosal brush biopsy testing of the inferior turbinate to detect local, 
      antigen-specific immunoglobulin E.
PG  - 69-74
LID - 10.1002/alr.20103 [doi]
AB  - BACKGROUND: This study investigated the ability of mucosal brush biopsy testing 
      (MBBT) from the inferior turbinate to detect local, antigen-specific 
      immunoglobulin E (IgE) in patients with allergic rhinitis, and these results were 
      then compared to skin prick testing (SPT). METHODS: A standard cytology brush was 
      used to harvest epithelial cells from the inferior turbinates of 20 patients who 
      tested positive to 2 or more airborne antigens out of a panel of 9 on SPT (N = 
      20). These cells were lysed and the resulting solution was tested for total and 
      antigen-specific IgE using immunofluorescence. RESULTS: Antigen-specific IgE for 
      at least 1 antigen was detected in 15 (75%) patients on MBBT. A statistically 
      significant association overall between the results of MBBT and SPT was present 
      (p = 0.01), but no significant associations were seen when examining individual 
      antigens. Patients with positive tests on MBBT had higher amounts of IgE and 
      total protein in biopsy samples than patients without positive tests on MBBT. The 
      mean wheal diameter in tests that were positive on MBBT were significantly larger 
      than the mean wheal diameter in tests that were negative on MBBT (p < .0001). 
      Eight tests were positive on MBBT that were negative on SPT in the same patients. 
      CONCLUSION: This study demonstrates for the first time that antigen-specific IgE 
      can be detected using MBBT in the sinonasal cavity. MBBT is more likely to be 
      positive in higher SPT grades and in biopsy samples with more protein present. 
      MBBT is also able to detect local IgE that is not detected on SPT.
CI  - Copyright (c) 2011 American Rhinologic Society-American Academy of Otolaryngic 
      Allergy, LLC.
FAU - Reisacher, William R
AU  - Reisacher WR
AD  - Department of Otolaryngology-Head and Neck Surgery, Weill Cornell Medical 
      College, New York, NY 10021, USA. wir2011@med.cornell.edu
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111108
PL  - United States
TA  - Int Forum Allergy Rhinol
JT  - International forum of allergy & rhinology
JID - 101550261
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Allergens/immunology
MH  - Biopsy/methods
MH  - Feasibility Studies
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/immunology/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Nasal Mucosa/metabolism
MH  - Rhinitis, Allergic, Perennial/immunology/*pathology
MH  - Skin Tests/methods
MH  - Turbinates/metabolism/*pathology
EDAT- 2012/02/09 06:00
MHDA- 2012/05/19 06:00
CRDT- 2012/02/08 06:00
PHST- 2011/08/22 00:00 [received]
PHST- 2011/09/28 00:00 [revised]
PHST- 2011/10/09 00:00 [accepted]
PHST- 2012/02/08 06:00 [entrez]
PHST- 2012/02/09 06:00 [pubmed]
PHST- 2012/05/19 06:00 [medline]
AID - 10.1002/alr.20103 [doi]
PST - ppublish
SO  - Int Forum Allergy Rhinol. 2012 Jan-Feb;2(1):69-74. doi: 10.1002/alr.20103. Epub 
      2011 Nov 8.