PMID- 22311926
OWN - NLM
STAT- MEDLINE
DCOM- 20120510
LR  - 20211021
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 80
IP  - 4
DP  - 2012 Apr
TI  - Strain-specific regulatory role of eukaryote-like serine/threonine phosphatase in 
      pneumococcal adherence.
PG  - 1361-72
LID - 10.1128/IAI.06311-11 [doi]
AB  - Streptococcus pneumoniae exploits a battery of virulence factors to colonize the 
      host. Although the eukaryote-like Ser/Thr kinase of S. pneumoniae (StkP) has been 
      implicated in physiology and virulence, the role of its cotranscribing 
      phosphatase (PhpP) has remained elusive. The construction of nonpolar markerless 
      phpP knockout mutants (DeltaphpP) in two pathogenic strains, D39 (type 2) and 
      6A-EF3114 (type 6A), indicated that PhpP is not indispensable for pneumococcal 
      survival. Further, PhpP also participates in the regulation of cell wall 
      biosynthesis/division, adherence, and biofilm formation in a strain-specific 
      manner. Additionally, we provide hitherto-unknown in vitro and in vivo evidence 
      of a physiologically relevant biochemical link between the StkP/PhpP-mediated 
      cognate regulation and the two-component regulatory system TCS06 (RR06/HK06) that 
      regulates the expression of the gene encoding an important pneumococcal surface 
      adhesin, CbpA, which was found to be significantly upregulated in DeltaphpP mutants. 
      In particular, StkP (threonine)-phosphorylated RR06 bound to the cbpA promoter 
      with high efficiency even in the absence of the HK06-responsive and catalytically 
      active aspartate 51 residue. Together, our findings unravel the significant 
      contributions of PhpP in pneumococcal physiology and adherence.
FAU - Agarwal, Shivangi
AU  - Agarwal S
AD  - Department of Pathology, The Ohio State University College of Medicine, Columbus, 
      Ohio, USA.
FAU - Agarwal, Shivani
AU  - Agarwal S
FAU - Pancholi, Preeti
AU  - Pancholi P
FAU - Pancholi, Vijay
AU  - Pancholi V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120206
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Bacterial Proteins)
RN  - 0 (CbpA protein, bacteria)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
SB  - IM
MH  - *Bacterial Adhesion
MH  - Bacterial Proteins/biosynthesis/genetics/*metabolism
MH  - Biofilms/growth & development
MH  - Cell Line
MH  - Gene Expression Regulation, Bacterial
MH  - Gene Knockout Techniques
MH  - Humans
MH  - Phosphoprotein Phosphatases/genetics/*metabolism
MH  - Signal Transduction
MH  - Streptococcus pneumoniae/*enzymology/genetics/pathogenicity/physiology
PMC - PMC3318399
EDAT- 2012/02/09 06:00
MHDA- 2012/05/11 06:00
PMCR- 2012/10/01
CRDT- 2012/02/08 06:00
PHST- 2012/02/08 06:00 [entrez]
PHST- 2012/02/09 06:00 [pubmed]
PHST- 2012/05/11 06:00 [medline]
PHST- 2012/10/01 00:00 [pmc-release]
AID - IAI.06311-11 [pii]
AID - 06311-11 [pii]
AID - 10.1128/IAI.06311-11 [doi]
PST - ppublish
SO  - Infect Immun. 2012 Apr;80(4):1361-72. doi: 10.1128/IAI.06311-11. Epub 2012 Feb 6.