PMID- 22312694 OWN - NLM STAT- MEDLINE DCOM- 20120320 LR - 20161125 IS - 0031-7144 (Print) IS - 0031-7144 (Linking) VI - 66 IP - 12 DP - 2011 Dec TI - Simultaneous determination of evodiamine and rutecarpine in rabbit plasma by LC-ESI-MS and its application to pharmacokinetics. PG - 920-3 AB - A sensitive and selective liquid chromatography-mass spectrometry (LC-MS) method for the determination of evodiamine and rutecarpine in rabbit plasma was developed and validated. The analytes and internal standard (IS) are extracted from plasma by one-step protein precipitation with acetonitrile, and separated on a Zorbax SB-C18 column (2.1 mm x 50 mm, 3.5 microm) using acetonitrile-0.1% formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode, and selective ion monitoring (SIM) mode used to quantify evodiamine and rutecarpine. The assay is linear over the range of 2-1600 ng/mL for evodiamine and rutecarpine, with a lower limit of quantification (LLOQ) of 5 ng/mL both for evodiamine and rutecarpine. Intra-day and inter-day precision are less than 12% and the accuracy are in the range of 90.9-104.3%. Furthermore, the newly developed method is successfully used for the determination of evodiamine and rutecarpine in rabbit plasma for pharmacokinetic study. FAU - Lin, Chongliang AU - Lin C AD - The First Affiliated Hospital, Wenzhou Medical College, Wenzhou, China. FAU - Pan, Xiaojun AU - Pan X FAU - Li, Wei AU - Li W FAU - Ma, Jianshe AU - Ma J FAU - Pan, Jingye AU - Pan J FAU - Cai, Jinzhang AU - Cai J FAU - Wang, Xianqin AU - Wang X FAU - Lin, Guanyang AU - Lin G LA - eng PT - Journal Article PL - Germany TA - Pharmazie JT - Die Pharmazie JID - 9800766 RN - 0 (Indicators and Reagents) RN - 0 (Indole Alkaloids) RN - 0 (Quinazolines) RN - 0 (Solutions) RN - 8XZV289PRY (rutecarpine) RN - C01825BVNL (evodiamine) SB - IM MH - Animals MH - Area Under Curve MH - Calibration MH - Chromatography, High Pressure Liquid MH - Half-Life MH - Indicators and Reagents MH - Indole Alkaloids/*blood/*pharmacokinetics MH - Injections, Intravenous MH - Male MH - Quinazolines/*blood/*pharmacokinetics MH - Rabbits MH - Reference Standards MH - Reproducibility of Results MH - Solutions MH - Spectrometry, Mass, Electrospray Ionization EDAT- 2012/02/09 06:00 MHDA- 2012/03/21 06:00 CRDT- 2012/02/09 06:00 PHST- 2012/02/09 06:00 [entrez] PHST- 2012/02/09 06:00 [pubmed] PHST- 2012/03/21 06:00 [medline] PST - ppublish SO - Pharmazie. 2011 Dec;66(12):920-3.