PMID- 22316433
OWN - NLM
STAT- MEDLINE
DCOM- 20130122
LR  - 20120803
IS  - 1532-3129 (Electronic)
IS  - 0021-9975 (Linking)
VI  - 147
IP  - 2-3
DP  - 2012 Aug-Oct
TI  - Pathogenesis of Korean type 1 (European genotype) porcine reproductive and 
      respiratory syndrome virus in experimentally infected pigs.
PG  - 275-84
LID - 10.1016/j.jcpa.2011.12.010 [doi]
AB  - The aim of this study was to elucidate the pathogenesis of experimental infection 
      with Korean type 1 porcine reproductive and respiratory syndrome virus (PRRSV) by 
      defining the virus distribution, sites of viral replication, viraemia and gross 
      and microscopical lesions in conventional pigs studied for 28 days after 
      intranasal inoculation. Mean rectal temperature was significantly higher in 
      infected pigs than in negative control pigs at 2 days post inoculation (dpi) 
      (P=0.004), 3 dpi (P<0.001), 4 dpi (P=0.003) and 5 dpi (P=0.034). The 
      log(10)TCID(50)/ml of type 1 PRRSV increased significantly at 0-1 dpi (P=0.024) 
      and 5-7 dpi (P=0.029), but decreased at 10-14 dpi (P=0.026) and 14-21 dpi 
      (P=0.012) in infected pigs. Infected pigs developed multifocal, tan-mottled areas 
      of lung tissue with irregular and indistinct borders. Microscopical lesions, when 
      present, were multifocal, mild to moderate, generally most extensive at 5-7 dpi 
      (P=0.036), and were nearly resolved at 28 dpi. Type 1 PRRSV nucleic acid and 
      antigen were detected exclusively within the cytoplasm of macrophages and type I 
      and II pneumocytes. The score for PRRSV-positive cells increased at 3-7 dpi 
      (P<0.05) and decreased at 10-14 dpi (P=0.034) in infected pigs. Thus, respiratory 
      disease was reproduced in conventional pigs by infection with Korean type 1 
      PRRSV.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Han, K
AU  - Han K
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
FAU - Seo, H W
AU  - Seo HW
FAU - Oh, Y
AU  - Oh Y
FAU - Kang, I
AU  - Kang I
FAU - Park, C
AU  - Park C
FAU - Chae, C
AU  - Chae C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120207
PL  - England
TA  - J Comp Pathol
JT  - Journal of comparative pathology
JID - 0102444
RN  - 0 (Antigens, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/analysis
MH  - Body Temperature
MH  - Disease Models, Animal
MH  - Female
MH  - Host-Pathogen Interactions
MH  - Lung/pathology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*pathology/virology
MH  - Porcine respiratory and reproductive syndrome virus/isolation & 
      purification/*physiology
MH  - RNA, Viral/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Swine
MH  - Swine Diseases/immunology/*pathology/virology
MH  - Time Factors
MH  - Virus Replication
EDAT- 2012/02/10 06:00
MHDA- 2013/01/23 06:00
CRDT- 2012/02/10 06:00
PHST- 2011/09/26 00:00 [received]
PHST- 2011/11/22 00:00 [revised]
PHST- 2011/12/22 00:00 [accepted]
PHST- 2012/02/10 06:00 [entrez]
PHST- 2012/02/10 06:00 [pubmed]
PHST- 2013/01/23 06:00 [medline]
AID - S0021-9975(11)00381-1 [pii]
AID - 10.1016/j.jcpa.2011.12.010 [doi]
PST - ppublish
SO  - J Comp Pathol. 2012 Aug-Oct;147(2-3):275-84. doi: 10.1016/j.jcpa.2011.12.010. 
      Epub 2012 Feb 7.