PMID- 22318348
OWN - NLM
STAT- MEDLINE
DCOM- 20121113
LR  - 20161026
IS  - 1827-191X (Electronic)
IS  - 0021-9509 (Linking)
VI  - 53
IP  - 5
DP  - 2012 Oct
TI  - Expression of interleukin-18 in a rat model of deep vein thrombosis.
PG  - 625-30
AB  - AIM: Interleukin-18 (IL-18) is an important proinflammatory cytokine. However, 
      little is known about the roles of IL-18 in the process of venous thrombosis. 
      This study aimed to investigate the roles of IL-18 during deep vein thrombosis 
      (DVT). METHODS: Fifty rats were randomly divided into 0 (control group), 12, 24, 
      36 and 48 h groups (10 rats in each group) by observation time. The inferior vena 
      cava (IVC) was ligated to establish the DVT model. Serum samples were extracted 
      to determine the levels of IL-18, tumor necrosis factor-alpha (TNF-alpha), 
      thromboxane B2 (TXB2) and 6-keto-prostaglandin Fl alpha (6-keto-PG Flalpha) by 
      enzyme-linked immunosorbent assay (ELISA). The weight and length of IVC was also 
      measured. RESULTS: The DVT model was successfully established by ligating IVC. 
      The injury of vein endothelium was observed in the model groups. IL-18, TNF-alpha, 
      TXB2, TXB2/6-keto-PG Flalpha levels and thrombus weight were significantly increased 
      in the model groups as compared with the control group, and peaked at 24 h after 
      IVC ligation. 6-keto-PG F1alpha slightly decreased in the model groups comparing with 
      the control group. IL-18 was positively correlated with TNF-alpha, TXB2, 
      TXB2/6-keto-PG Flalpha ratio and thrombus weight. However, IL-18 was negatively 
      correlated with 6-keto-PG Flalpha. There was a positive correlation between 
      TXB2/6-keto-PG Flalpha ratio and thrombus weight. CONCLUSION: Serum IL-18 level 
      increased in the process of DVT, which might impair venous endothelial cells and 
      result in venous thrombosis. IL-18 might be a new potential therapeutic target of 
      DVT prevention.
FAU - Mo, J
AU  - Mo J
AD  - Department of Orthopedics Surgery, the First Affiliated Hospital of Guangzhou 
      Medical College, Guangdong Province, PR China.
FAU - Bai, B
AU  - Bai B
FAU - Li, Y
AU  - Li Y
FAU - Deng, J
AU  - Deng J
FAU - Zhang, S
AU  - Zhang S
FAU - Chen, Y
AU  - Chen Y
FAU - Zeng, Y
AU  - Zeng Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120208
PL  - Italy
TA  - J Cardiovasc Surg (Torino)
JT  - The Journal of cardiovascular surgery
JID - 0066127
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-18)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 54397-85-2 (Thromboxane B2)
RN  - 58962-34-8 (6-Ketoprostaglandin F1 alpha)
SB  - IM
MH  - 6-Ketoprostaglandin F1 alpha/blood
MH  - Animals
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Inflammation Mediators/*blood
MH  - Interleukin-18/*blood
MH  - Ligation
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Thromboxane B2/blood
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/blood
MH  - Up-Regulation
MH  - Vena Cava, Inferior/*immunology/pathology/surgery
MH  - Venous Thrombosis/etiology/*immunology/pathology
EDAT- 2012/02/10 06:00
MHDA- 2012/11/14 06:00
CRDT- 2012/02/10 06:00
PHST- 2012/02/10 06:00 [entrez]
PHST- 2012/02/10 06:00 [pubmed]
PHST- 2012/11/14 06:00 [medline]
AID - R37126504 [pii]
PST - ppublish
SO  - J Cardiovasc Surg (Torino). 2012 Oct;53(5):625-30. Epub 2012 Feb 8.