PMID- 22320165
OWN - NLM
STAT- MEDLINE
DCOM- 20130903
LR  - 20181202
IS  - 1439-0396 (Electronic)
IS  - 0931-2439 (Linking)
VI  - 97
IP  - 2
DP  - 2013 Apr
TI  - Hepatoprotective effects of S-adenosylmethionine and silybin on canine 
      hepatocytes in vitro.
PG  - 331-41
LID - 10.1111/j.1439-0396.2012.01275.x [doi]
AB  - Inflammation and oxidative stress are associated with liver injury and 
      development of liver disease. The transcription factors nuclear factor-kappa beta 
      (NF-kappaB) and nuclear factor erythroid 2-related factor 2 (Nrf2) play critical 
      roles in modulating liver injury and damage. Activation of NF-kappaB induces 
      production of pro-inflammatory molecules including prostaglandin E2 (PGE2 ), 
      interleukin-8 (IL-8) and macrophage chemotactic protein-1 (MCP-1). Nrf2 regulates 
      genes controlling antioxidants. Our laboratory previously showed that 
      hepatocytes, the primary functional cell type comprising liver tissue, respond to 
      the cytokine interleukin-1 beta (IL-1beta) by increased production of PGE2 , IL-8 
      and MCP-1. This increase is associated with nuclear translocation of NF-kappaB. In 
      this study, we evaluated whether primary canine hepatocytes pre-treated with the 
      combination of S-adenosylmethionine (SAMe; 30 and 2000 ng/ml) and silybin (SB; 
      298 ng/ml), agents with known anti-inflammatory and antioxidant properties, could 
      attenuate IL-1beta-induced inflammation and oxidative stress. The SAMe and SB 
      combination reduced cytokine-induced PGE2 , IL-8 and MCP-1 production while also 
      inhibiting NF-kappaB nuclear translocation. These changes were accompanied by 
      increased antioxidant enzyme-reduced glutathione (GSH) comparable to control 
      levels. The study shows for the first time that the SAMe and SB combination 
      inhibits both inflammation and oxidative stress through two separate signalling 
      pathways.
CI  - (c) 2012 Blackwell Verlag GmbH.
FAU - Au, A Y
AU  - Au AY
AD  - Research and Development, Nutramax Laboratories, Inc., 2208 Lakeside Blvd., 
      Edgewood, MD 21040, USA.
FAU - Hasenwinkel, J M
AU  - Hasenwinkel JM
FAU - Frondoza, C G
AU  - Frondoza CG
LA  - eng
PT  - Journal Article
DEP - 20120209
PL  - Germany
TA  - J Anim Physiol Anim Nutr (Berl)
JT  - Journal of animal physiology and animal nutrition
JID - 101126979
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-8)
RN  - 0 (Silymarin)
RN  - 4RKY41TBTF (Silybin)
RN  - 7LP2MPO46S (S-Adenosylmethionine)
RN  - GAN16C9B8O (Glutathione)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Antioxidants/*pharmacology
MH  - Biomarkers
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/metabolism
MH  - Dinoprostone/genetics/metabolism
MH  - Dogs
MH  - Gene Expression Regulation/drug effects
MH  - Glutathione/metabolism
MH  - Hepatocytes/cytology/*drug effects
MH  - Interleukin-1beta/pharmacology
MH  - Interleukin-8/genetics/metabolism
MH  - Oxidative Stress
MH  - S-Adenosylmethionine/*pharmacology
MH  - Silybin
MH  - Silymarin/*pharmacology
EDAT- 2012/02/11 06:00
MHDA- 2013/09/04 06:00
CRDT- 2012/02/11 06:00
PHST- 2012/02/11 06:00 [entrez]
PHST- 2012/02/11 06:00 [pubmed]
PHST- 2013/09/04 06:00 [medline]
AID - 10.1111/j.1439-0396.2012.01275.x [doi]
PST - ppublish
SO  - J Anim Physiol Anim Nutr (Berl). 2013 Apr;97(2):331-41. doi: 
      10.1111/j.1439-0396.2012.01275.x. Epub 2012 Feb 9.