PMID- 22320435
OWN - NLM
STAT- MEDLINE
DCOM- 20121206
LR  - 20220331
IS  - 1937-3392 (Electronic)
IS  - 1937-3384 (Print)
IS  - 1937-3384 (Linking)
VI  - 18
IP  - 8
DP  - 2012 Aug
TI  - A microwell cell culture platform for the aggregation of pancreatic beta-cells.
PG  - 583-92
LID - 10.1089/ten.TEC.2011.0504 [doi]
AB  - Cell-cell contact between pancreatic beta-cells is important for maintaining 
      survival and normal insulin secretion. Various techniques have been developed to 
      promote cell-cell contact between beta-cells, but a simple yet robust method that 
      affords precise control over three-dimensional (3D) beta-cell cluster size has not 
      been demonstrated. To address this need, we developed a poly(ethylene glycol) 
      (PEG) hydrogel microwell platform using photolithography. This microwell 
      cell-culture platform promotes the formation of 3D beta-cell aggregates of defined 
      sizes from 25 to 210 mum in diameter. Using this platform, mouse insulinoma 6 
      (MIN6) beta-cells formed aggregates with cell-cell adherin junctions. These 
      naturally formed cell aggregates with controllable sizes can be removed from the 
      microwells for macroencapsulation, implantation, or other biological assays. When 
      removed and subsequently encapsulated in PEG hydrogels, the aggregated cell 
      clusters demonstrated improved cellular viability (>90%) over 7 days in culture, 
      while the beta-cells encapsulated as single cells maintained only 20% viability. 
      Aggregated MIN6 cells also exhibited more than fourfold higher insulin secretion 
      in response to a glucose challenge compared with encapsulated single beta-cells. 
      Further, the cell aggregates stained positively for E-cadherin, indicative of the 
      formation of cell junctions. Using this hydrogel microwell cell-culture method, 
      viable and functional beta-cell aggregates of specific sizes were created, providing 
      a platform from which other biologically relevant questions may be answered.
FAU - Bernard, Abigail B
AU  - Bernard AB
AD  - Department of Chemical and Biological Engineering, University of Colorado, 
      Boulder, CO 80309, USA.
FAU - Lin, Chien-Chi
AU  - Lin CC
FAU - Anseth, Kristi S
AU  - Anseth KS
LA  - eng
GR  - R01DK076084/DK/NIDDK NIH HHS/United States
GR  - Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120319
PL  - United States
TA  - Tissue Eng Part C Methods
JT  - Tissue engineering. Part C, Methods
JID - 101466663
RN  - 0 (Cadherins)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Hydrogels)
RN  - 0 (Insulin)
RN  - 0 (Polymers)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - Animals
MH  - Cadherins/metabolism
MH  - Cell Communication
MH  - Cell Culture Techniques/*methods
MH  - Cell Survival
MH  - Cross-Linking Reagents/chemistry
MH  - Hydrogels/chemistry
MH  - Insulin/metabolism
MH  - Insulin-Secreting Cells/*cytology
MH  - Mice
MH  - Microscopy, Confocal/methods
MH  - Microscopy, Fluorescence/methods
MH  - Polyethylene Glycols/chemistry
MH  - Polymers/chemistry
PMC - PMC3401388
EDAT- 2012/02/11 06:00
MHDA- 2012/12/10 06:00
PMCR- 2012/03/16
CRDT- 2012/02/11 06:00
PHST- 2012/02/11 06:00 [entrez]
PHST- 2012/02/11 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
PHST- 2012/03/16 00:00 [pmc-release]
AID - 10.1089/ten.tec.2011.0504 [pii]
AID - 10.1089/ten.TEC.2011.0504 [doi]
PST - ppublish
SO  - Tissue Eng Part C Methods. 2012 Aug;18(8):583-92. doi: 10.1089/ten.TEC.2011.0504. 
      Epub 2012 Mar 19.