PMID- 22320865
OWN - NLM
STAT- MEDLINE
DCOM- 20120913
LR  - 20240321
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Print)
IS  - 1044-5498 (Linking)
VI  - 31
IP  - 6
DP  - 2012 Jun
TI  - Inhibition of mammalian target of rapamycin signaling by CCI-779 (temsirolimus) 
      induces growth inhibition and cell cycle arrest in Cashmere goat fetal 
      fibroblasts (Capra hircus).
PG  - 1095-9
LID - 10.1089/dna.2011.1559 [doi]
AB  - The mammalian target of rapamycin (mTOR) is a Ser/Thr kinase. It plays an 
      evolutionarily conserved role in regulating cell growth, proliferation, survival, 
      and metabolism via different cellular processes. The purpose of this study was to 
      explore the inhibitory effects of CCI-779 (temsirolimus), a specific mTOR 
      inhibitor, on mTOR signaling, and examine the mechanism of cell growth 
      suppression by CCI-779 in Cashmere goat fetal fibroblasts (GFb cells). GFb cells 
      were sensitive to CCI-779 and the survival rate of cells treated with >3.0 muM of 
      CCI-779 was significantly reduced compared with the control (p<0.01). CCI-779 
      inhibited the phosphorylation of mTOR (at Ser2448) and S6 (at Ser240/244), and 
      the expression of mTOR, p70S6K, and S6. Thus, CCI-779 was toxic to GFb cells, and 
      it induced a dose-dependent decrease in cell proliferation and caused G1/S cell 
      cycle arrest. Taken together, these data show that CCI-779 can inhibit mTOR 
      signaling and proliferation in GFb cells in vitro. Therefore, mTOR is an 
      important regulator for GFb cell growth and proliferation.
FAU - Wang, Zhigang
AU  - Wang Z
AD  - The Key Laboratory of Mammal Reproductive Biology and Biotechnology, College of 
      Life Science, Inner Mongolia University, Ministry of Education, Hohhot, PR China. 
      lswzg@imu.edu.cn
FAU - Liu, Taodi
AU  - Liu T
FAU - Chen, Yuhao
AU  - Chen Y
FAU - Zhang, Xin
AU  - Zhang X
FAU - Liu, Mingtao
AU  - Liu M
FAU - Fu, Haixia
AU  - Fu H
FAU - Liu, Dongjun
AU  - Liu D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120209
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (Protein Kinase Inhibitors)
RN  - 624KN6GM2T (temsirolimus)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Cell Cycle Checkpoints/*drug effects
MH  - Enzyme Activation/drug effects
MH  - *Fetus
MH  - Fibroblasts/*cytology/drug effects/metabolism
MH  - G1 Phase Cell Cycle Checkpoints/drug effects
MH  - *Goats
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - S Phase Cell Cycle Checkpoints/drug effects
MH  - Signal Transduction/*drug effects
MH  - Sirolimus/*analogs & derivatives/pharmacology
MH  - TOR Serine-Threonine Kinases/antagonists & inhibitors/*metabolism
PMC - PMC3378953
EDAT- 2012/02/11 06:00
MHDA- 2012/09/14 06:00
PMCR- 2013/06/01
CRDT- 2012/02/11 06:00
PHST- 2012/02/11 06:00 [entrez]
PHST- 2012/02/11 06:00 [pubmed]
PHST- 2012/09/14 06:00 [medline]
PHST- 2013/06/01 00:00 [pmc-release]
AID - 10.1089/dna.2011.1559 [pii]
AID - 10.1089/dna.2011.1559 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2012 Jun;31(6):1095-9. doi: 10.1089/dna.2011.1559. Epub 2012 Feb 
      9.