PMID- 22320974
OWN - NLM
STAT- MEDLINE
DCOM- 20120628
LR  - 20170308
IS  - 2476-762X (Electronic)
IS  - 1513-7368 (Linking)
VI  - 12
IP  - 10
DP  - 2011
TI  - Crocin inhibits proliferation and nucleic acid synthesis and induces apoptosis in 
      the human tongue squamous cell carcinoma cell line Tca8113.
PG  - 2679-83
AB  - BACKGROUND: Cancer chemoprevention is a proven effective strategy for oral 
      squamous cell carcinoma (OSCC). The present study was designed to investigate the 
      effects of crocin, a potential chemopreventive agent, on growth and DNA and RNA 
      content in a human tongue squamous cell carcinoma cell line, Tca8113. METHODS: 
      Tca8113 cells were treated with crocin for 24, 48, 72, and 96 h at concentrations 
      of 0.1, 0.2, 0.4, and 0.8 mM. Tumor cell viability was investigated using the 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. In 
      addition, Tca8113 cells were treated with 0.4 mM crocin and cytotoxic effects as 
      an inducer of apoptosis were analyzed using flow cytometry. Furthermore, acridine 
      orange (AO) staining and observation using laser scanning confocal microscopy 
      (LSCM) were used to determine the effects of the drug on nucleic acid synthesis. 
      RESULTS: Crocin decreased Tca8113 cell viability and growth remarkably at 24, 48, 
      72, and 96 h, in a concentration-dependent manner (P<0.05). In addition, 0.4 mM 
      crocin significantly induced both early and late apoptosis of Tca8113 cells. 
      Moreover, the cellular DNA and RNA content was significantly downregulated by 0.4 
      mM crocin compared with the negative control (P<0.01). CONCLUSIONS: Our 
      observations support the feasibility of applying crocin as a chemoprophylactic 
      agent and treatment for OSCCs.
FAU - Sun, Jun
AU  - Sun J
AD  - State Key Laboratory of Oral Diseases, West China Hospital, Sichuan University, 
      Chengdu, China. acomnet@126.com
FAU - Xu, Xiao-meng
AU  - Xu XM
FAU - Ni, Chen-zhong
AU  - Ni CZ
FAU - Zhang, Hong
AU  - Zhang H
FAU - Li, Xiao-yu
AU  - Li XY
FAU - Zhang, Chao-liang
AU  - Zhang CL
FAU - Liu, Yu-rong
AU  - Liu YR
FAU - Li, Sheng-fu
AU  - Li SF
FAU - Zhou, Qi-zhi
AU  - Zhou QZ
FAU - Zhou, Hong-mei
AU  - Zhou HM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Thailand
TA  - Asian Pac J Cancer Prev
JT  - Asian Pacific journal of cancer prevention : APJCP
JID - 101130625
RN  - 0 (Antineoplastic Agents)
RN  - 36-88-4 (Carotenoids)
RN  - 63231-63-0 (RNA)
RN  - 877GWI46C2 (crocin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Apoptosis/*drug effects
MH  - Carcinoma, Squamous Cell/metabolism/*pathology
MH  - Carotenoids/*pharmacology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects
MH  - Chemoprevention/methods
MH  - DNA/biosynthesis
MH  - Humans
MH  - RNA/biosynthesis
MH  - Tongue Neoplasms/metabolism/*pathology
EDAT- 2012/02/11 06:00
MHDA- 2012/06/29 06:00
CRDT- 2012/02/11 06:00
PHST- 2012/02/11 06:00 [entrez]
PHST- 2012/02/11 06:00 [pubmed]
PHST- 2012/06/29 06:00 [medline]
PST - ppublish
SO  - Asian Pac J Cancer Prev. 2011;12(10):2679-83.