PMID- 22323190
OWN - NLM
STAT- MEDLINE
DCOM- 20130426
LR  - 20221207
IS  - 1099-1565 (Electronic)
IS  - 0958-0344 (Linking)
VI  - 23
IP  - 6
DP  - 2012 Nov-Dec
TI  - Tandem solid phase extraction followed by online trapping-hydrophilic interaction 
      chromatography-tandem mass spectrometry for sensitive detection of endogenous 
      cytokinins in plant tissues.
PG  - 559-68
LID - 10.1002/pca.2353 [doi]
AB  - INTRODUCTION: Cytokinins (CKs) are a group of plant hormones that play pivotal 
      roles at low concentration in plant growth, development and regulatory pathways. 
      In order to study the function, metabolism and signal transduction of CKs, high 
      performance analytical techniques are required for determination of their 
      endogenous levels. OBJECTIVE: To develop a highly sensitive, selective and 
      reliable method for identification and quantification of CKs by employing a 
      tandem solid phase extraction (SPE)-online trapping-hydrophilic interaction 
      chromatography (HILIC)-MS/MS method. MATERIAL AND METHODS: The extraction was 
      performed firstly with tandem SPE containing a C(18) cartridge and a silica@C(8) 
      /SO(3) H cartridge. After CKs were eluted from the silica@C(8)/SO(3) H cartridge, 
      the desorption solvent was concentrated and redissolved in H(2)O and then 
      injected into the online trapping-HILIC-MS/MS system with (Poly(MAA-co-EGDMA)) 
      monolith as the trapping column. Subsequently, trapping, washing, desorption, 
      separation and detection were accomplished automatically on the system. RESULTS: 
      Good linearities were obtained for eight cytokinins with correlation coefficients 
      (R(2)) > 0.9964. The limits of detection (LOD; S:N = 3) for the targets ranged 
      from 0.042 to 1.6 pg/mL. Reproducibility of the method was evaluated with 
      intraday and interday relative standard deviations (RSDs) less than 13.4% and the 
      recoveries ranged from 77.3% to 116.3%. The results showed that the LOD of the 
      analytical method were at least one order of magnitude lower compared with other 
      previously reported methods. Furthermore, only 20 mg of plant tissues were 
      required for the quantitative analysis of the major CKs, which is, to the best of 
      our knowledge, the smallest amount reported so far for the determination of 
      endogenous CKs in plant tissues. CONCLUSION: The tandem SPE-online 
      trapping-HILIC-MS/MS method developed in current study provides a powerful tool 
      for the convenient and highly sensitive quantification of the major CKs in plant 
      tissue.
CI  - Copyright (c) 2012 John Wiley & Sons, Ltd.
FAU - Liu, Zhao
AU  - Liu Z
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China.
FAU - Yuan, Bi-Feng
AU  - Yuan BF
FAU - Feng, Yu-Qi
AU  - Feng YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120209
PL  - England
TA  - Phytochem Anal
JT  - Phytochemical analysis : PCA
JID - 9200492
RN  - 0 (Cytokinins)
RN  - 0 (Plant Growth Regulators)
SB  - IM
MH  - Arabidopsis/chemistry
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/instrumentation/methods
MH  - Chromatography, Liquid/methods
MH  - Cytokinins/*analysis
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Limit of Detection
MH  - Solanum lycopersicum/chemistry
MH  - Oryza/chemistry
MH  - Plant Growth Regulators/analysis
MH  - Plant Leaves/chemistry
MH  - Plants/*chemistry
MH  - Reproducibility of Results
MH  - Seedlings/chemistry
MH  - Sensitivity and Specificity
MH  - Solid Phase Extraction/*methods
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2012/02/11 06:00
MHDA- 2013/04/27 06:00
CRDT- 2012/02/11 06:00
PHST- 2011/10/20 00:00 [received]
PHST- 2012/01/11 00:00 [revised]
PHST- 2012/01/12 00:00 [accepted]
PHST- 2012/02/11 06:00 [entrez]
PHST- 2012/02/11 06:00 [pubmed]
PHST- 2013/04/27 06:00 [medline]
AID - 10.1002/pca.2353 [doi]
PST - ppublish
SO  - Phytochem Anal. 2012 Nov-Dec;23(6):559-68. doi: 10.1002/pca.2353. Epub 2012 Feb 
      9.