PMID- 22325058
OWN - NLM
STAT- MEDLINE
DCOM- 20121108
LR  - 20231213
IS  - 1744-313X (Electronic)
IS  - 1744-3121 (Linking)
VI  - 39
IP  - 4
DP  - 2012 Aug
TI  - Association of polymorphisms in interleukin-18 and interleukin-28B with hepatitis 
      B recurrence after liver transplantation in Chinese Han population.
PG  - 346-52
LID - 10.1111/j.1744-313X.2012.01097.x [doi]
AB  - Interleukin-18 (IL-18) is a potent proinflammatory cytokine, which can promote 
      hepatitis B virus clearance. The latest studies find that genetic polymorphisms 
      near the IL-28B gene are strongly associated with sustained viral response and 
      spontaneous viral clearance in patients with chronically infected hepatitis C and 
      hepatitis B. We investigated the effect of rs187238 and rs1946518 in IL-18 gene 
      and rs8099917 in IL-28B gene on HBV recurrence in liver transplant patients. A 
      total of 200 liver transplant recipients and relevant donors were enrolled in 
      this study. The patients' mean follow-up was 39 month (range 10-65 month). All 
      liver transplant recipients were in a stable stage. The total recipients (n = 
      200) were divided into end-stage liver disease secondary to hepatitis B (n = 140) 
      and end-stage liver disease secondary to other diseases (n = 60) before 
      transplantation. Recipients (n = 140) with hepatitis B before transplantation 
      were defined to nonHBV recurrence group (n = 119) or HBV recurrence group (n = 
      21), which was positive for HBsAg or elevatory in HBV DNA (>2.0 x 10(2) IU 
      mL(-1)) after transplantation. For the recipients (n = 140) had hepatitis B 
      before transplantation, we studied the single-nucleotide polymorphisms (SNPs) of 
      IL-18 gene (rs187238 and rs1946518) and IL-28B gene (rs8099917) by 
      high-resolution melting (HRM) curve analysis. The serum levels of IL-18 and IFN-gamma 
      were tested by ELISA. The serums levels of IFN-gamma were lower in HBV recurrence 
      group than that in nonHBV recurrence group (P < 0.01). The genotype of IL-28B 
      gene rs8099917 was associated with alanine aminotransferase (ALT) levels and 
      aspartate aminotransferase (AST) levels in HBV-related liver transplant 
      recipients (n = 140). The recipients with allele G (GG+GT) had higher ALT and AST 
      levels (P < 0.05). No association was found between IL-18 gene and IL-28B gene 
      polymorphisms with HBV recurrence in the liver transplant recipients or the 
      donors. We identified that the IFN-gamma was a protective factor of HBV recurrence 
      after liver transplantation. The allele G of rs8099917 was associated with 
      hepatitis B-related hepatocytes injury. The rs8099917 G allele subgroup should 
      reinforce antiviral therapy.
CI  - (c) 2012 Blackwell Publishing Ltd.
FAU - Li, Y
AU  - Li Y
AD  - Department of Clinical Immunological Laboratory, West China Hospital, Sichuan 
      University, Chengdu, China.
FAU - Shi, Y
AU  - Shi Y
FAU - Chen, J
AU  - Chen J
FAU - Cai, B
AU  - Cai B
FAU - Ying, B
AU  - Ying B
FAU - Wang, L
AU  - Wang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120210
PL  - England
TA  - Int J Immunogenet
JT  - International journal of immunogenetics
JID - 101232337
RN  - 0 (DNA, Viral)
RN  - 0 (Hepatitis B Surface Antigens)
RN  - 0 (interferon-lambda, human)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukins)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9008-11-1 (Interferons)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 2.6.1.2 (Alanine Transaminase)
SB  - IM
MH  - Aged
MH  - Alanine Transaminase/blood
MH  - Alleles
MH  - Asian People/*genetics
MH  - Aspartate Aminotransferases/blood
MH  - Case-Control Studies
MH  - China/epidemiology
MH  - DNA, Viral/blood
MH  - Female
MH  - Follow-Up Studies
MH  - Gene Frequency
MH  - Genetic Association Studies
MH  - Genetic Predisposition to Disease
MH  - Genome, Human
MH  - Hepatitis B/*genetics/virology
MH  - Hepatitis B Surface Antigens/blood
MH  - Hepatitis B virus/pathogenicity
MH  - Humans
MH  - Interferon-gamma/blood
MH  - Interferons
MH  - Interleukin-18/*genetics
MH  - Interleukins/*genetics
MH  - Liver Transplantation/ethnology/*pathology
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Single Nucleotide
MH  - Promoter Regions, Genetic
MH  - Recurrence
EDAT- 2012/02/14 06:00
MHDA- 2012/11/09 06:00
CRDT- 2012/02/14 06:00
PHST- 2012/02/14 06:00 [entrez]
PHST- 2012/02/14 06:00 [pubmed]
PHST- 2012/11/09 06:00 [medline]
AID - 10.1111/j.1744-313X.2012.01097.x [doi]
PST - ppublish
SO  - Int J Immunogenet. 2012 Aug;39(4):346-52. doi: 10.1111/j.1744-313X.2012.01097.x. 
      Epub 2012 Feb 10.