PMID- 22325218
OWN - NLM
STAT- MEDLINE
DCOM- 20121126
LR  - 20200205
IS  - 0253-3766 (Print)
IS  - 0253-3766 (Linking)
VI  - 33
IP  - 8
DP  - 2011 Aug
TI  - [Epithelial-mesenchymal transition (EMT) and its effect on microRNA expression in 
      lung cancer].
PG  - 590-3
AB  - OBJECTIVE: To investigate the effect of epithelial-mesenchymal transition (EMT) 
      on the expression of microRNAs (miRNAs) in lung cancer A549 cells. METHODS: 
      Transforming growth factor beta-1 (TGF-beta 1) in different concentrations was 
      used to induce EMT in lung cancer A549 cells. The morphological changes were 
      observed under phase-contrast microscope. The changes of EMT-related proteins 
      were analyzed by Western blot. The changes of miRNAs expression after EMT were 
      detected by microRNA (miRNA) array. Real time quantitative RT-PCR was applied to 
      verify the reliability of miRNA array results. RESULTS: The lung cancer A549 
      cells became elongated and the cell-cell junction became loose after EMT. The 
      epithelial protein marker E-cadherin was down-regulated and the mesenchymal 
      protein markers vimentin and fibronectin up-regulated. There were 51 miRNAs 
      showing statistically significant changes of expression more than double (P<0.05) 
      after EMT. Among them 18 were up-regulated and 33 down-regulated. Of them, 
      mir-33a was down-regulated by 92.8% and mir-193a-3p by 86.5%. Real time 
      quantitative RT-PCR showed that mir-33a was down-regulated by 73.1% and 
      mir-193a-3p by 56.6%. CONCLUSION: Epithelial-mesenchymal transition has effects 
      on the expression of miRNAs, and miRNAs may regulate the invasion and metastasis 
      of lung cancer cells via EMT.
FAU - Wang, Hai-Bing
AU  - Wang HB
AD  - Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University 
      School of Medicine, Shanghai 200433, China.
FAU - Zhang, Hui-Jun
AU  - Zhang HJ
FAU - Su, Jin-Mei
AU  - Su JM
FAU - Zhou, Wen-Yong
AU  - Zhou WY
FAU - Wang, He-Yong
AU  - Wang HY
FAU - Chen, Xiao-Feng
AU  - Chen XF
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Zhong Liu Za Zhi
JT  - Zhonghua zhong liu za zhi [Chinese journal of oncology]
JID - 7910681
RN  - 0 (Cadherins)
RN  - 0 (Fibronectins)
RN  - 0 (MIRN193 microRNA, human)
RN  - 0 (MIRN33a microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Vimentin)
SB  - IM
MH  - Adenocarcinoma/genetics/*metabolism/pathology
MH  - Adenocarcinoma of Lung
MH  - Cadherins/metabolism
MH  - Cell Line, Tumor
MH  - *Epithelial-Mesenchymal Transition/drug effects
MH  - Fibronectins/metabolism
MH  - Gene Expression Profiling
MH  - Humans
MH  - Lung Neoplasms/genetics/*metabolism/pathology
MH  - MicroRNAs/genetics/*metabolism
MH  - Transforming Growth Factor beta1/administration & dosage/pharmacology
MH  - Vimentin/metabolism
EDAT- 2012/02/14 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/02/14 06:00
PHST- 2012/02/14 06:00 [entrez]
PHST- 2012/02/14 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
PST - ppublish
SO  - Zhonghua Zhong Liu Za Zhi. 2011 Aug;33(8):590-3.