PMID- 22326902
OWN - NLM
STAT- MEDLINE
DCOM- 20120713
LR  - 20120319
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 30
IP  - 14
DP  - 2012 Mar 23
TI  - Murine CD8(+)T cell cytotoxicity against schistosomula induced by inoculation of 
      schistosomal 22.6/26GST coupled Sepharose 4B beads.
PG  - 2440-7
LID - 10.1016/j.vaccine.2012.01.068 [doi]
AB  - Schistosomasis is a world-wide parasitic disease. Although chemotherapy is the 
      main treatment method for schistosomasis currently, it cannot prevent schistosome 
      reinfection. Up to now no effective vaccine is available to prevent 
      schistosomiasis. Dendritic cells (DCs) are one of the key players in the cellular 
      immune response and play an important role in antigen presentation as 
      antigen-presenting cells. Here we reported a novel large particulate antigen, in 
      which Sepharose 4B beads were coated with Sj22.6/26GST. Our results showed that 
      this particulate antigen could be cross-presented by DCs to CD8(+)T cells. 
      Furthermore, CD8(+)T cells stimulated by particulate antigen directly exerted 
      cytotoxicity against Schistosoma japonicum schistosomula. We also demonstrated 
      that S. japonicum schistosomula acquired the MHC class I molecules from host 
      blood serum and presented the molecules at the larval surface. While it may help 
      them escape from the host immune surveillance, these MHC I-antigen complexes 
      presented on the surface render schistosomula the potential targets of the 
      CD8(+)T cell cytotoxicity induced by particulate antigen-based vaccine. Finally 
      we evaluated the protective immunity of this particulate vaccine in a mouse 
      infection challenge model. Our data clearly showed that the particulate vaccine 
      induced a partial reduction in both worm burdens and egg loads. Taken together, 
      these results suggest that this large particulate vaccine could be a potential 
      vaccine for the prevention of schistosome infection.
CI  - Copyright A(c) 2012 Elsevier Ltd. All rights reserved.
FAU - Zhou, Ying
AU  - Zhou Y
AD  - Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 
      210029, PR China.
FAU - Zhang, Hui
AU  - Zhang H
FAU - Sun, Xin-Juan
AU  - Sun XJ
FAU - Zheng, Dan
AU  - Zheng D
FAU - Liang, Yue-Jin
AU  - Liang YJ
FAU - Luo, Jie
AU  - Luo J
FAU - Wang, Yong
AU  - Wang Y
FAU - Zhang, Zhao-Song
AU  - Zhang ZS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120210
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antigens, Helminth)
RN  - 0 (Helminth Proteins)
RN  - 0 (Sj22.6-26GST vaccine)
RN  - 0 (Vaccines)
RN  - 9007-81-2 (Freund's Adjuvant)
RN  - 9012-36-6 (Sepharose)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/immunology
MH  - Antigens, Helminth/administration & dosage/*immunology
MH  - Cytotoxicity, Immunologic/*immunology
MH  - Dendritic Cells/immunology
MH  - Female
MH  - Freund's Adjuvant
MH  - Helminth Proteins/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Schistosoma japonicum/*immunology
MH  - Schistosomiasis/*immunology/prevention & control
MH  - Sepharose/chemistry
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Vaccines/*immunology
EDAT- 2012/02/14 06:00
MHDA- 2012/07/14 06:00
CRDT- 2012/02/14 06:00
PHST- 2011/09/01 00:00 [received]
PHST- 2012/01/16 00:00 [revised]
PHST- 2012/01/21 00:00 [accepted]
PHST- 2012/02/14 06:00 [entrez]
PHST- 2012/02/14 06:00 [pubmed]
PHST- 2012/07/14 06:00 [medline]
AID - S0264-410X(12)00106-5 [pii]
AID - 10.1016/j.vaccine.2012.01.068 [doi]
PST - ppublish
SO  - Vaccine. 2012 Mar 23;30(14):2440-7. doi: 10.1016/j.vaccine.2012.01.068. Epub 2012 
      Feb 10.