PMID- 22327909
OWN - NLM
STAT- MEDLINE
DCOM- 20120521
LR  - 20120410
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 136
IP  - 3
DP  - 2012
TI  - Molecular insights into X;BTA5 chromosome rearrangements in the tribe Antilopini 
      (Bovidae).
PG  - 188-98
LID - 10.1159/000336248 [doi]
AB  - For a clade that includes Antilope, Gazella,Nanger and Eudorcas (Antilopinae), 
      X;BTA5 translocation is a synapomorphy. Using a combination of fluorescence in 
      situ hybridization (FISH) probes and polymerase chain reaction techniques, we 
      provide (i) the first insight into the X;BTA5 architecture which differs in the 
      species under study: Antilope cervicapra (genus Antilope), Gazella leptoceros 
      (genus Gazella) and Nanger dama ruficollis (genus Nanger), (ii) determination of 
      interstitial satellite DNA at the X;BTA5 junctions, and (iii) determination of 
      repetitive sequences occupying constitutive heterochromatin of Xp arms in the 
      studied species. The distribution of 2 repetitive DNA families in the centromeric 
      regions of all chromosomes has been investigated by FISH with probes representing 
      satellite I and satellite II DNA in all studied species. In this context, we 
      discuss a markedly smaller centromere in the BTA5 (Y2) unfused chromosomes in 
      males in the XY1Y2 determining system in comparison with other acrocentrics. An 
      analysis of karyotypic data described in current published studies revealed a 
      disparity with the data determined by FISH. In this report, we document 
      chromosomal fusions in the 3 species mentioned resulting from FISH with painting 
      probes prepared from cattle (Bos taurus). The number and chromosomal location of 
      nucleolus organizer regions were determined by FISH. In the present study, we 
      emphasize the importance of chromosomal rearrangement verification, particularly, 
      if they are used for phylogenetic analysis.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - Cernohorska, H
AU  - Cernohorska H
AD  - Veterinary Research Institute, Brno, Czech Republic.
FAU - Kubickova, S
AU  - Kubickova S
FAU - Vahala, J
AU  - Vahala J
FAU - Rubes, J
AU  - Rubes J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120211
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - Animals
MH  - Antelopes/*genetics
MH  - Chromosome Painting
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Nucleolus Organizer Region
MH  - Polymerase Chain Reaction
MH  - Translocation, Genetic
MH  - *X Chromosome
EDAT- 2012/02/14 06:00
MHDA- 2012/05/23 06:00
CRDT- 2012/02/14 06:00
PHST- 2011/12/09 00:00 [accepted]
PHST- 2012/02/14 06:00 [entrez]
PHST- 2012/02/14 06:00 [pubmed]
PHST- 2012/05/23 06:00 [medline]
AID - 000336248 [pii]
AID - 10.1159/000336248 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2012;136(3):188-98. doi: 10.1159/000336248. Epub 2012 Feb 
      11.