PMID- 22328258
OWN - NLM
STAT- MEDLINE
DCOM- 20121121
LR  - 20120720
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 167
IP  - 5
DP  - 2012 Jul
TI  - Volatiles formation from grape must fermentation using a cryophilic and 
      thermotolerant yeast.
PG  - 1183-98
LID - 10.1007/s12010-012-9582-2 [doi]
AB  - Grape must fermentation performance and volatiles formation by simultaneously 
      cryophilic and thermotolerant yeast (strain AXAZ-1), isolated from grapes in 
      Greece, was evaluated in a wide temperature range (5-40 degrees C). Yeast strain was 
      immobilized on brewer's spent grains (BSG) and the formed biocatalyst was 
      introduced into a Multi-Stage Fixed Bed Tower (MFBT) bioreactor. Almost complete 
      sugar utilization from the aforementioned biocatalyst was observed in a wide 
      temperature spectrum, ranging from 5  degrees C to 37  degrees C, while at 40  degrees C residual sugar 
      was up to 29 g/l. Time to complete fermentation with the immobilized yeast ranged 
      from 290 h at 5  degrees C and 120 h at 40  degrees C to 25 h at 33  degrees C. The daily ethanol 
      productivity reached maximum (88.6 g/l) and minimum (5.6 g/l) levels at 33  degrees C and 
      5  degrees C, respectively. The aroma-related compounds' profiles of immobilized cells at 
      different fermentation temperatures were evaluated by using solid phase 
      microextraction (SPME) gas chromatography/mass spectrometry (GC/MS). Must 
      fermentation resulted in a high-quality fermentation product due to the low 
      concentrations of higher and amyl alcohols at all temperatures tested. AXAZ-1 is 
      a very promising strain for quality wine production, as it is capable of 
      performing fermentations of high ethanol concentration and productivities in both 
      low and high temperatures.
FAU - Kopsahelis, Nikolaos
AU  - Kopsahelis N
AD  - Food Biotechnology Group, Section of Analytical Environmental and Applied 
      Chemistry, Department of Chemistry, University of Patras, 26500 Patras, Greece. 
      Kopsahelis@upatras.gr
FAU - Bosnea, Loulouda
AU  - Bosnea L
FAU - Kanellaki, Maria
AU  - Kanellaki M
FAU - Koutinas, Athanasios A
AU  - Koutinas AA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120211
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Volatile Organic Compounds)
SB  - IM
MH  - Beverages/*microbiology
MH  - Bioreactors/microbiology
MH  - Cells, Immobilized/metabolism
MH  - *Cold Temperature
MH  - *Fermentation
MH  - *Hot Temperature
MH  - Saccharomyces cerevisiae/cytology/*metabolism/physiology
MH  - *Vitis
MH  - Volatile Organic Compounds/*metabolism
EDAT- 2012/02/14 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/02/14 06:00
PHST- 2011/11/30 00:00 [received]
PHST- 2012/01/20 00:00 [accepted]
PHST- 2012/02/14 06:00 [entrez]
PHST- 2012/02/14 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 10.1007/s12010-012-9582-2 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2012 Jul;167(5):1183-98. doi: 10.1007/s12010-012-9582-2. 
      Epub 2012 Feb 11.