PMID- 22328380
OWN - NLM
STAT- MEDLINE
DCOM- 20120611
LR  - 20120213
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 845
DP  - 2012
TI  - Murine bone marrow-derived dendritic cells and T-cell activation by Candida 
      albicans.
PG  - 261-75
LID - 10.1007/978-1-61779-539-8_17 [doi]
AB  - Dendritic cells (DCs) detect and respond to microbes or their components by 
      producing cytokines and other molecules that can activate the proliferation and 
      differentiation pathways of T cells. Investigation of DC responses to pathogens 
      would thus provide important insights into how T-cell responses most appropriate 
      for the pathogen are induced. Here, we describe methods for the use of mixed 
      leukocyte reactions, to determine the proliferative and cytokine responses of 
      murine splenic T cells in response to co-culture with bone marrow-derived DCs 
      stimulated with Candida albicans.
FAU - Gibson, Joanne
AU  - Gibson J
AD  - School of Medicine and Dentistry, University of Aberdeen, Aberdeen, UK.
FAU - Gow, Neil A R
AU  - Gow NA
FAU - Wong, Simon Y C
AU  - Wong SY
LA  - eng
GR  - 08088/Wellcome Trust/United Kingdom
GR  - Biotechnology and Biological Sciences Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/immunology
MH  - Candida albicans/*immunology
MH  - Cell Proliferation
MH  - Coculture Techniques
MH  - Cytokines/immunology
MH  - Dendritic Cells/*cytology/*immunology
MH  - *Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes/*immunology
EDAT- 2012/02/14 06:00
MHDA- 2012/06/12 06:00
CRDT- 2012/02/14 06:00
PHST- 2012/02/14 06:00 [entrez]
PHST- 2012/02/14 06:00 [pubmed]
PHST- 2012/06/12 06:00 [medline]
AID - 10.1007/978-1-61779-539-8_17 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2012;845:261-75. doi: 10.1007/978-1-61779-539-8_17.