PMID- 2232849
OWN - NLM
STAT- MEDLINE
DCOM- 19901204
LR  - 20190824
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 14
IP  - 9
DP  - 1990
TI  - A study of multidrug resistance and cell kinetics in a child with near-haploid 
      acute lymphoblastic leukemia.
PG  - 771-7
AB  - Marked hypodiploidy is found in a small group of patients with acute 
      lymphoblastic leukemia (ALL) and is associated with very poor prognosis. Cells 
      from a patient with near-haploid ALL (karyotype: 27 XY, DNA index = 0.5) were 
      investigated by multiparameter flow cytometry at relapse and at multiple 
      time-points during reinduction chemotherapy. The cell cycle of these near-haploid 
      leukemic blasts and their chromatin structure was studied by acridine orange (AO) 
      DNA/RNA flow cytometric assays. Most leukemic cells were in "G0", and no 
      recruitment of the bone marrow cells into the G1 phase of the cell cycle was 
      found during reinduction therapy with high dose cytosine arabinoside. After two 
      cycles of chemotherapy, the patient achieved clinical remission, but persistent 
      haploid cells were identified by flow cytometry and he relapsed after 8 weeks and 
      died after 16.7 weeks. The leukemic blasts expressed very high levels of a 180 kd 
      p-glycoprotein associated with multidrug resistance and daunomycin efflux could 
      be blocked by verapamil. Expression of gp 180 and the verapamil effect on 
      intracellular daunomycin concentration indicate multidrug resistance. We conclude 
      that cell kinetic quiescence and multidrug resistance may both be factors 
      responsible for the poor prognosis of this patient with near-haploid ALL. Further 
      studies of this patient group should determine if these mechanisms are indeed 
      responsible for the poor prognosis associated with near-haploid leukemia.
FAU - Redner, A
AU  - Redner A
AD  - Department of Pediatrics, Memorial Sloan-Kettering Cancer Center, New York, NY 
      10021.
FAU - Hegewisch, S
AU  - Hegewisch S
FAU - Haimi, J
AU  - Haimi J
FAU - Steinherz, P
AU  - Steinherz P
FAU - Jhanwar, S
AU  - Jhanwar S
FAU - Andreeff, M
AU  - Andreeff M
LA  - eng
GR  - CA-38980/CA/NCI NIH HHS/United States
GR  - CA-41305/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (elastin microfibril interface located protein)
RN  - CJ0O37KU29 (Verapamil)
RN  - ZS7284E0ZP (Daunorubicin)
SB  - IM
MH  - Cell Cycle
MH  - Chromosome Aberrations
MH  - Daunorubicin/pharmacokinetics
MH  - *Drug Resistance
MH  - Haplotypes
MH  - Humans
MH  - Membrane Glycoproteins/analysis
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics/*pathology
MH  - Verapamil/pharmacology
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1016/0145-2126(90)90070-p [doi]
PST - ppublish
SO  - Leuk Res. 1990;14(9):771-7. doi: 10.1016/0145-2126(90)90070-p.