PMID- 22328528
OWN - NLM
STAT- MEDLINE
DCOM- 20130124
LR  - 20120528
IS  - 1477-9137 (Electronic)
IS  - 0021-9533 (Linking)
VI  - 125
IP  - Pt 8
DP  - 2012 Apr 15
TI  - Binding of the ERalpha and ARNT1 AF2 domains to exon 21 of the SRC1 isoform SRC1e is 
      essential for estrogen- and dioxin-related transcription.
PG  - 2004-16
LID - 10.1242/jcs.097246 [doi]
AB  - Steroid receptor co-activator 1 (SRC1) is a transcriptional co-activator of 
      numerous transcription factors involving nuclear receptors. Aryl hydrocarbon 
      receptor nuclear translocator 1 (ARNT1) is an obligatory transcriptional partner 
      of the aryl hydrocarbon receptor (AhR) and hypoxia inducible factor-1alpha (HIF-1alpha), 
      as well as a co-activator of estrogen receptors (ERs). To initiate transcription, 
      the activation function 2 (AF2) domains of estrogen-activated ERs interact with 
      LxxLL motifs in the nuclear receptor interaction domain (NID) of SRC1. Here we 
      describe an estrogen and LxxLL domain-independent ERalpha AF2 binding to SRC1e exon 
      21. In addition, we found an AF2 domain in exon 16 of ARNT1 that also binds to 
      SRC1e exon 21. Surprisingly, the interaction between SRC1e exon 21 and the AF2 
      domain of ERalpha functions as a crucial enhancer of estrogen-induced transcription. 
      The binding of ARNT1 AF2 to SRC1e exon 21 enhances the transcriptional response 
      to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), but the upregulation essentially 
      depends on two cyclin destruction boxes (D-boxes), which are also located on exon 
      16 of ARNT1. Our findings reveal that a binding site for ERalpha and ARNT1 AF2 
      domains in the C-terminus of SRC1e upregulates estrogen- and TCDD-related 
      responses in mammalian cells.
FAU - Endler, Alexander
AU  - Endler A
AD  - Department of Regenerative Medicine, and Stem Cell Research Center, Tongji 
      University School of Medicine, Shanghai 200092, China. alexanderendler@msn.com
FAU - Chen, Li
AU  - Chen L
FAU - Zhang, Jun
AU  - Zhang J
FAU - Xu, Guo-Tong
AU  - Xu GT
FAU - Shibasaki, Futoshi
AU  - Shibasaki F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120210
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (ARNT protein, human)
RN  - 0 (Dioxins)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogens)
RN  - 0 (Protein Isoforms)
RN  - 138391-32-9 (Aryl Hydrocarbon Receptor Nuclear Translocator)
RN  - EC 2.3.1.48 (NCOA1 protein, human)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
SB  - IM
MH  - Aryl Hydrocarbon Receptor Nuclear Translocator/*chemistry/genetics/*metabolism
MH  - Dioxins/*metabolism
MH  - Estrogen Receptor alpha/genetics/*metabolism
MH  - Estrogens/*metabolism
MH  - Exons
MH  - Gene Expression Regulation
MH  - HeLa Cells
MH  - Humans
MH  - MCF-7 Cells
MH  - Nuclear Receptor Coactivator 1/*chemistry/genetics/*metabolism
MH  - Protein Binding
MH  - Protein Isoforms/chemistry/genetics/metabolism
MH  - Protein Structure, Tertiary
MH  - Transcription, Genetic
MH  - *Up-Regulation
EDAT- 2012/02/14 06:00
MHDA- 2013/01/25 06:00
CRDT- 2012/02/14 06:00
PHST- 2012/02/14 06:00 [entrez]
PHST- 2012/02/14 06:00 [pubmed]
PHST- 2013/01/25 06:00 [medline]
AID - jcs.097246 [pii]
AID - 10.1242/jcs.097246 [doi]
PST - ppublish
SO  - J Cell Sci. 2012 Apr 15;125(Pt 8):2004-16. doi: 10.1242/jcs.097246. Epub 2012 Feb 
      10.