PMID- 22328910
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1876-3901 (Print)
IS  - 1876-3901 (Electronic)
VI  - 2
DP  - 2010
TI  - 'Chromosomal Rainbows' Detect Oncogenic Rearrangements of Signaling Molecules in 
      Thyroid Tumors.
PG  - 13-22
AB  - Altered signal transduction can be considered a hallmark of many solid tumors. In 
      thyroid cancers the receptor tyrosine kinase (rtk) genes NTRK1 (Online Mendelian 
      Inheritance in Man = OMIM *191315, also known as 'TRKA'), RET ('Rearranged during 
      Transfection protooncogene', OMIM *164761) and MET (OMIM *164860) have been 
      reported as activated, rearranged or overexpressed. In many cases, a combination 
      of cytogenetic and molecular techniques allows elucidation of cellular changes 
      that initiate tumor development and progression. While the mechanisms leading to 
      overexpression of the rtk MET gene remain largely unknown, a variety of 
      chromosomal rearrangements of the RET or NTKR1 gene could be demonstrated in 
      thyroid cancer. Abnormal expressions in these tumors seem to follow a similar 
      pattern: the rearrangement translocates the 3'- end of the rtk gene including the 
      entire catalytic domain to an expressed gene leading to a chimeric RNA and 
      protein with kinase activity. Our research was prompted by an increasing number 
      of reports describing translocations involving ret and previously unknown 
      translocation partners.We developed a high resolution technique based on 
      fluorescence in situ hybridization (FISH) to allow rapid screening for 
      cytogenetic rearrangements which complements conventional chromosome banding 
      analysis. Our technique applies simultaneous hybridization of numerous probes 
      labeled with different reporter molecules which are distributed along the target 
      chromosome allowing the detection of cytogenetic changes at near megabasepair 
      (Mbp) resolution. Here, we report our results using a probe set specific for 
      human chromosome 10, which is altered in a significant portion of human thyroid 
      cancers (TC's). While rendering accurate information about the cytogenetic 
      location of rearranged elements, our multi-locus, multi-color analysis was 
      developed primarily to overcome limitations of whole chromosome painting (WCP) 
      and chromosome banding techniques for fine mapping of breakpoints in papillary 
      thyroid cancer (PTC).
FAU - O'Brien, Benjamin
AU  - O'Brien B
AD  - Life Sciences Division, E.O. Lawrence Berkeley National Laboratory, Berkeley, CA 
      94720, USA.
FAU - Jossart, Gregg H
AU  - Jossart GH
FAU - Ito, Yuko
AU  - Ito Y
FAU - Greulich-Bode, Karin M
AU  - Greulich-Bode KM
FAU - Weier, Jingly F
AU  - Weier JF
FAU - Munne, Santiago
AU  - Munne S
FAU - Clark, Orlo H
AU  - Clark OH
FAU - Weier, Heinz-Ulrich G
AU  - Weier HU
LA  - eng
GR  - R21 CA123370-02/CA/NCI NIH HHS/United States
GR  - R01 CA136685/CA/NCI NIH HHS/United States
GR  - R21 CA123370/CA/NCI NIH HHS/United States
GR  - R01 HD045736/HD/NICHD NIH HHS/United States
GR  - R01 CA136685-03/CA/NCI NIH HHS/United States
GR  - R01 HD045736-03/HD/NICHD NIH HHS/United States
GR  - R01 CA080792-01A2/CA/NCI NIH HHS/United States
PT  - Journal Article
PL  - Netherlands
TA  - Open Cell Signal J
JT  - The open cell signaling journal
JID - 101555349
PMC - PMC3276079
MID - NIHMS265714
EDAT- 2010/01/01 00:00
MHDA- 2010/01/01 00:01
PMCR- 2012/02/09
CRDT- 2012/02/14 06:00
PHST- 2012/02/14 06:00 [entrez]
PHST- 2010/01/01 00:00 [pubmed]
PHST- 2010/01/01 00:01 [medline]
PHST- 2012/02/09 00:00 [pmc-release]
AID - 10.2172/1011038 [doi]
PST - ppublish
SO  - Open Cell Signal J. 2010;2:13-22. doi: 10.2172/1011038.