PMID- 22330602
OWN - NLM
STAT- MEDLINE
DCOM- 20120629
LR  - 20131121
IS  - 1873-2976 (Electronic)
IS  - 0960-8524 (Linking)
VI  - 110
DP  - 2012 Apr
TI  - Sodium hydroxide pretreatment of genetically modified switchgrass for improved 
      enzymatic release of sugars.
PG  - 364-70
LID - 10.1016/j.biortech.2012.01.097 [doi]
AB  - Overcoming biomass recalcitrance to bioconversion is crucial for cellulosic 
      biofuels commercialization. In this study, Alamo switchgrass (Panicum virgatum 
      L.) was genetically transformed to suppress the expression of 4-coumarate-CoA 
      ligase (4CL). The transgenic plants were determined to have lignin content 
      reductions of up to 5.8%. The ratios of acid soluble lignin (ASL) to acid 
      insoluble lignin (AIL) and syringyl/guaiacyl (S/G) in transgenic plants were 
      21.4-64.3% and 11.8-164.5%, respectively, higher than those of conventional 
      biomass. Both conventional and transgenic plants were pretreated with 0.5%, 1%, 
      and 2% (w/v) NaOH for 15, 30, and 60min at 121 degrees C, followed by enzymatic 
      hydrolysis with commercial cellulases and xylanases. At the optimal conditions, 
      the glucan and xylan conversion efficiency in the best transgenic plants were 16% 
      and 18% higher than the conventional plant, respectively. The results show that 
      down-regulation of 4CL gene promoted enzymatic hydrolysis of plant cell walls 
      following a mild alkali pretreatment.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Wang, Ziyu
AU  - Wang Z
AD  - Department of Biological and Agricultural Engineering, North Carolina State 
      University, Raleigh, NC 27695-7625, USA.
FAU - Li, Ruyu
AU  - Li R
FAU - Xu, Jiele
AU  - Xu J
FAU - Marita, Jane M
AU  - Marita JM
FAU - Hatfield, Ronald D
AU  - Hatfield RD
FAU - Qu, Rongda
AU  - Qu R
FAU - Cheng, Jay J
AU  - Cheng JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120128
PL  - England
TA  - Bioresour Technol
JT  - Bioresource technology
JID - 9889523
RN  - 55X04QC32I (Sodium Hydroxide)
SB  - IM
MH  - Biomass
MH  - *Carbohydrate Metabolism
MH  - Hydrolysis
MH  - *Plants, Genetically Modified
MH  - Poaceae/genetics/*metabolism
MH  - Sodium Hydroxide/*chemistry
EDAT- 2012/02/15 06:00
MHDA- 2012/06/30 06:00
CRDT- 2012/02/15 06:00
PHST- 2011/11/14 00:00 [received]
PHST- 2012/01/13 00:00 [revised]
PHST- 2012/01/18 00:00 [accepted]
PHST- 2012/02/15 06:00 [entrez]
PHST- 2012/02/15 06:00 [pubmed]
PHST- 2012/06/30 06:00 [medline]
AID - S0960-8524(12)00121-6 [pii]
AID - 10.1016/j.biortech.2012.01.097 [doi]
PST - ppublish
SO  - Bioresour Technol. 2012 Apr;110:364-70. doi: 10.1016/j.biortech.2012.01.097. Epub 
      2012 Jan 28.