PMID- 22332739
OWN - NLM
STAT- MEDLINE
DCOM- 20121001
LR  - 20181201
IS  - 1470-8744 (Electronic)
IS  - 0885-4513 (Linking)
VI  - 59
IP  - 1
DP  - 2012 Jan-Feb
TI  - siRNA high-throughput kinase library screen identifies protein kinase, 
      DNA-activated catalytic polypeptide to play a role in MyD88-induced IFNA2 
      activation and IL-8 secretion.
PG  - 6-14
LID - 10.1002/bab.66 [doi]
AB  - The discovery of RNA interference has led to the development of short interfering 
      RNA (siRNA) screening, which has been widely used to study biological pathways. 
      Here, we describe the development and validation of a system suitable to identify 
      cellular genes involved in interferon A2 (IFNA2) promoter activation and 
      interleukin (IL)-8 secretion downstream of MyD88. Forty genes were identified. 
      Five genes were selected for further study. One gene, protein kinase, 
      DNA-activated catalytic polypeptide (PRKDC), was confirmed to play a role in 
      MyD88-induced IFNA2 activation and IL-8 secretion.
CI  - Copyright (c) 2012 International Union of Biochemistry and Molecular Biology, Inc.
FAU - Charlaftis, Nikolaos
AU  - Charlaftis N
AD  - Biopharm Discovery, Medicines Research Centre, GlaxoSmithKline, Stevenage, 
      Hertfordshire, UK. n.charlaftis@imperial.ac.uk
FAU - Fearon, Douglas T
AU  - Fearon DT
FAU - Schoenemeyer, Annett
AU  - Schoenemeyer A
FAU - Morley, Peter J
AU  - Morley PJ
LA  - eng
GR  - Biotechnology and Biological Sciences Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120123
PL  - United States
TA  - Biotechnol Appl Biochem
JT  - Biotechnology and applied biochemistry
JID - 8609465
RN  - 0 (IFNA2 protein, human)
RN  - 0 (Interferon-alpha)
RN  - 0 (Interleukin-8)
RN  - 0 (MYD88 protein, human)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.11.1 (DNA-Activated Protein Kinase)
RN  - EC 2.7.11.1 (PRKDC protein, human)
SB  - IM
MH  - Cell Survival
MH  - DNA-Activated Protein Kinase/genetics/*metabolism
MH  - Gene Library
MH  - HEK293 Cells
MH  - Humans
MH  - Interferon-alpha/*genetics
MH  - Interleukin-8/*metabolism
MH  - Myeloid Differentiation Factor 88/*metabolism
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - RNA, Small Interfering/genetics
MH  - Up-Regulation/genetics
EDAT- 2012/02/16 06:00
MHDA- 2012/10/02 06:00
CRDT- 2012/02/16 06:00
PHST- 2011/05/16 00:00 [received]
PHST- 2011/11/28 00:00 [accepted]
PHST- 2012/02/16 06:00 [entrez]
PHST- 2012/02/16 06:00 [pubmed]
PHST- 2012/10/02 06:00 [medline]
AID - 10.1002/bab.66 [doi]
PST - ppublish
SO  - Biotechnol Appl Biochem. 2012 Jan-Feb;59(1):6-14. doi: 10.1002/bab.66. Epub 2012 
      Jan 23.