PMID- 22333174
OWN - NLM
STAT- MEDLINE
DCOM- 20120618
LR  - 20140226
IS  - 0578-1426 (Print)
IS  - 0578-1426 (Linking)
VI  - 50
IP  - 12
DP  - 2011 Dec
TI  - [The cytogenetic and molecular genetic study of 81 multiple myeloma patients].
PG  - 1039-42
AB  - OBJECTIVE: To explore the cytogenetic characteristics of multiple myeloma (MM) 
      patients, to evaluate the effect of a long-term culture stimulated by cytokines 
      on cytogenetic study of MM, and to investigate the clinical detection value of 
      RB1 and P53 deletion in interphase plasma cells by using fluorescence in situ 
      hybridization (FISH). METHODS: Karyotype analysis was performed in 81 MM patients 
      by using the short-term culture of bone marrow cell and G-banding technique. 
      Among the 81 MM patients, 28 patients used two culture methods: one was the 
      short-term culture and the other was to culture cells for 6 days with recombinant 
      granulocyte-macrophage colony-stimulating factor (GM-CSF) (40 microg/L) and IL-6 (10 
      microg/L). RB1 and P53 deletion were detected on interphase plasma cells by using 
      FISH in 31 patients. RESULTS: Among the 81 patients, 75 had enough metaphases for 
      analysis. Among the 75 patients, 31 (41.3%) had clonal karyotypic abnormalities 
      including 4 numeric abnormalities, 11 structural abnormalities and 16 both 
      abnormalities. Among the 28 patients using two culture methods, the clonal 
      karyotypic abnormalities were detected in 6 patients (25.0%) in the group of 
      cultured for 24 hours, and 14 patients (51.9%) in 6-day culture group with a 
      significant difference (P = 0.026). RB1 deletion and P53 deletion were detected 
      in 10 patients (32.3%) and 11 patients (35.5%), respectively, with both RB1 and 
      P53 deletions be detected in 5 patients (16.1%). CONCLUSIONS: More than half of 
      the tested MM patients have both numeric and structural chromosome abnormalities. 
      The karyotype analysis using banding technique is basic cytogenetic study. 
      Extended culture in the presence of IL-6 and GM-CSF could improve the efficiency 
      of cytogenetic analysis to MM. Interphase FISH is a sensitive method of clinical 
      application significance to detect the gene deletion of MM.
FAU - Li, Jiang-tao
AU  - Li JT
AD  - Department of Hematology, Beijing Hospital, Beijing, China.
FAU - Chang, Nai-bai
AU  - Chang NB
FAU - Liu, Hui
AU  - Liu H
FAU - Pei, Lei
AU  - Pei L
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Nei Ke Za Zhi
JT  - Zhonghua nei ke za zhi
JID - 16210490R
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Aberrations
MH  - *Chromosome Banding
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/diagnosis/*genetics
EDAT- 2012/02/16 06:00
MHDA- 2012/06/19 06:00
CRDT- 2012/02/16 06:00
PHST- 2012/02/16 06:00 [entrez]
PHST- 2012/02/16 06:00 [pubmed]
PHST- 2012/06/19 06:00 [medline]
PST - ppublish
SO  - Zhonghua Nei Ke Za Zhi. 2011 Dec;50(12):1039-42.