PMID- 22335102 OWN - NLM STAT- MEDLINE DCOM- 20120404 LR - 20190918 IS - 0273-1223 (Print) IS - 0273-1223 (Linking) VI - 64 IP - 8 DP - 2011 TI - The use of microsensors to study the role of the loading rate and surface velocity on the growth and the composition of nitrifying biofilms. PG - 1607-13 AB - The good composition and activity of biofilms are very important for successful operation and control of fixed-film biological reactors employed in liquid effluents treatment. During the last decade, microsensors have been applied to study microbial ecology. These sensors could provide information regarding the microbial activity concerning nitrification and denitrification that occur inside biofilms. Other techniques of molecular biology, such as fluorescence in situ hybridization (FISH), have also contributed to this matter because their application aids in the identification of the bacterial populations that compose the biofilms. The focus of this paper was to study the loading rate and surface velocity to promote the development of nitrifying biofilms in three distinct flow cells that were employed in the post treatment of a synthetic wastewater simulating the effluent from a UASB (Upflow Anaerobic Sludge Blanket) reactor. Using the FISH technique, it was found that the population of ammonia-oxidizing-bacteria was greater than that of nitrite-oxidizing-bacteria; this was also supported by the lower production of nitrate determined by physicochemical and microsensor analyses. It was verified that the loading rate and surface velocity that promoted the greatest nitrogen removal were 0.25 g N-amon m(-2)biofilm day(-1) and 1 m h(-1), respectively. FAU - Gonzalez, B C AU - Gonzalez BC AD - Department of Hydraulics and Sanitation, University of Sao Paulo (USP), Sao Carlos School of Engineering, Av. Trabalhador Sao-carlense, 400-Sao Carlos-SP, 13566-590, Brazil. beatrizgonzalez@sc.usp.br FAU - Spinola, A L G AU - Spinola AL FAU - Lamon, A W AU - Lamon AW FAU - Araujo, J C AU - Araujo JC FAU - Campos, J R AU - Campos JR LA - eng PT - Journal Article PL - England TA - Water Sci Technol JT - Water science and technology : a journal of the International Association on Water Pollution Research JID - 9879497 RN - N762921K75 (Nitrogen) RN - S88TT14065 (Oxygen) SB - IM MH - Bacteria/metabolism MH - *Biofilms MH - Bioreactors MH - In Situ Hybridization, Fluorescence MH - Nitrogen/chemistry/metabolism MH - Oxidation-Reduction MH - Oxygen MH - Waste Disposal, Fluid/*methods EDAT- 2012/02/18 06:00 MHDA- 2012/04/05 06:00 CRDT- 2012/02/17 06:00 PHST- 2012/02/17 06:00 [entrez] PHST- 2012/02/18 06:00 [pubmed] PHST- 2012/04/05 06:00 [medline] AID - 10.2166/wst.2011.716 [doi] PST - ppublish SO - Water Sci Technol. 2011;64(8):1607-13. doi: 10.2166/wst.2011.716.