PMID- 22335816 OWN - NLM STAT- MEDLINE DCOM- 20121227 LR - 20160511 IS - 1865-1682 (Electronic) IS - 1865-1674 (Linking) VI - 59 IP - 6 DP - 2012 Dec TI - Naturally co-infected boars with both porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. PG - 532-8 LID - 10.1111/j.1865-1682.2012.01313.x [doi] AB - In this study, the humoral response against porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2), the presence of the virus in semen and serum and the genetic characteristics of the virus detected in 15 boars from a commercial farm were analysed. The results showed that 53% of the boars presented anti-PRRSV antibodies and 100% presented anti-PCV2 antibodies. Porcine reproductive and respiratory syndrome virus was detected in 43% of the boars and 73% were positive to PCV2. The complete ORF5 gene of PRRSV of 14 samples and a fragment of the ORF2 gene of PCV2 of 22 samples were sequenced. Porcine reproductive and respiratory syndrome virus analysis revealed <92% identity in viruses from semen and serum of two boars, whereas in the rest of the boars the identity was >97.5%. As for PCV2, two boars presented an identity <95% in serum and semen and the rest had an identity >96%. The results showed that PRRSV- and PCV2-naturally infected boars can be found, and at least two different strains of viruses from semen and serum can be detected. CI - (c) 2012 Blackwell Verlag GmbH. FAU - Burgara-Estrella, A AU - Burgara-Estrella A AD - Laboratorio de Inmunologia, Centro de Investigacion en Alimentacion y Desarrollo, A.C., Hermosillo, Sonora, Mexico. FAU - Montalvo-Corral, M AU - Montalvo-Corral M FAU - Bolanos, A AU - Bolanos A FAU - Ramirez-Mendoza, H AU - Ramirez-Mendoza H FAU - Valenzuela, O AU - Valenzuela O FAU - Hernandez, J AU - Hernandez J LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120215 PL - Germany TA - Transbound Emerg Dis JT - Transboundary and emerging diseases JID - 101319538 RN - 0 (Antibodies, Viral) RN - 0 (DNA, Viral) RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Antibodies, Viral/blood MH - Circoviridae Infections/epidemiology/*veterinary/virology MH - Circovirus/genetics/immunology/isolation & purification MH - Coinfection/epidemiology/veterinary MH - DNA, Viral/analysis MH - Male MH - Porcine Reproductive and Respiratory Syndrome/*epidemiology MH - Porcine respiratory and reproductive syndrome virus/genetics/immunology/isolation & purification MH - Prevalence MH - RNA, Viral/analysis MH - Semen/virology MH - Swine MH - Swine Diseases/*epidemiology EDAT- 2012/02/18 06:00 MHDA- 2012/12/28 06:00 CRDT- 2012/02/17 06:00 PHST- 2012/02/17 06:00 [entrez] PHST- 2012/02/18 06:00 [pubmed] PHST- 2012/12/28 06:00 [medline] AID - 10.1111/j.1865-1682.2012.01313.x [doi] PST - ppublish SO - Transbound Emerg Dis. 2012 Dec;59(6):532-8. doi: 10.1111/j.1865-1682.2012.01313.x. Epub 2012 Feb 15.